Suri Fatemeh, Biginaloo Abdullah, Ghadimi Mehdi, Banadaki Hossein Dehghan, Paylakhi Seyyed Hassan, Kanavi Mozhgan Rezaei
Ophthalmic Research Center, Research Institute for Ophthalmology and Vision Science, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
School of Biology, Damghan University, Damghan, Iran.
Iran J Public Health. 2023 Dec;52(12):2630-2642. doi: 10.18502/ijph.v52i12.14324.
A disintegrin and metalloproteinase with thrombospondin motifs (ADAMTS) is a superfamily of extracellular proteinases found in both mammals and invertebrates. Although there is some evidence about the role of ADAMTSs in ocular diseases such as glaucoma and ectopia lentis, but there is little information about the expression patterns of and ADAMTS-like ( and ) genes in human ocular tissues. This study aimed to evaluate the expression profiling of (L) superfamily of genes in different ocular tissues based on age.
In 2019, nine human donated eye globes were provided from the Central Eye Bank of Iran, and were divided into three different groups based on age (under 3 yr old, between 20 to 50 and upper 50 yr old). To assess expression patterns of (L) genes in different ocular tissues including trabecular meshwork, lens, retinal pigment epithelium, macula, and optic nerve in the three age groups, total RNA was extracted from the tissues and reverse transcription polymerase chain reaction followed by Real-time PCR was performed.
We demonstrated not only each member of (L) superfamily shows different expression pattern between the five investigated ocular tissues, but also some members have differential expressions among the investigated age groups in same tissues.
Differential expression of (L) genes in ocular tissues from different age groups could explain some functional aspects of the tissues and also may be used as prognostic and diagnostic biomarkers for ocular diseases and pathologies. Further studies are required to explore their functional roles associated with ocular pathologies.
含血小板反应蛋白基序的解聚素和金属蛋白酶（ADAMTS）是在哺乳动物和无脊椎动物中均发现的细胞外蛋白酶超家族。尽管有一些证据表明ADAMTS在青光眼和晶状体异位等眼部疾病中发挥作用，但关于ADAMTS样基因（ADAMTS-L）在人眼组织中的表达模式的信息却很少。本研究旨在评估基于年龄的ADAMTS-L超家族基因在不同眼组织中的表达谱。
2019年，从伊朗中央眼库获得9个捐赠的人眼球，并根据年龄分为三个不同组（3岁以下、20至50岁之间和50岁以上）。为了评估三个年龄组中不同眼组织（包括小梁网、晶状体、视网膜色素上皮、黄斑和视神经）中ADAMTS-L基因的表达模式，从组织中提取总RNA，并进行逆转录聚合酶链反应，随后进行实时PCR。
我们证明，不仅ADAMTS-L超家族的每个成员在五个被研究的眼组织之间表现出不同的表达模式，而且一些成员在相同组织的被研究年龄组之间也有差异表达。
ADAMTS-L基因在不同年龄组眼组织中的差异表达可以解释组织的一些功能方面，也可作为眼部疾病和病理状况的预后和诊断生物标志物。需要进一步研究来探索它们与眼部病理相关的功能作用。
