濒危药用植物Rech.f.（）的离体微繁殖与保存：遗传稳定性、植物化学及生物学评估

In vitro micropropagation and conservation of endangered medicinal plant Rech.f. (): genetic fidelity, phytochemical and biological assessment.

作者信息

Zamani Mahdieh, Sonboli Ali, Goldansaz Mostafa, Mirjalili Mohammad Hossein

机构信息

Department of Agriculture, Medicinal Plants and Drugs Research Institute, Shahid Beheshti University, Tehran, 1983969411 Iran.

Department of Biology, Medicinal Plants and Drugs Research Institute, Shahid Beheshti University, Tehran, 1983969411 Iran.

出版信息

Physiol Mol Biol Plants. 2024 Jan;30(1):67-80. doi: 10.1007/s12298-024-01416-x. Epub 2024 Feb 23.

DOI:10.1007/s12298-024-01416-x

PMID:38435858

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10901756/

Abstract

UNLABELLED

An efficient in vitro protocol was introduced for the conservation of , a vulnerable and endangered medicinal species found in the central of Iran for the first time. Growth, phytochemical, and biological traits of in vitro regenerated plant (RP) and acclimated plant (AP) were compared to the mother plant (MP). In addition, the genetic stability of AP was assessed by using inter-simple sequence repeats (ISSR) markers. The highest number of lateral branches (4.25) was obtained from the medium with 3 mg/mL kinetin (KIN), while the highest length of lateral branches (13.25 cm) was achieved on the medium culture fortified with 3 mg/mL thidiazuron (TDZ) and 6-benzylaminopurine (BAP). The highest number of leaves (20.25) and main branch length (12.25 cm) were obtained from the medium containing 3 mg/mL TDZ. The highest number of roots (46.25) and root length (2.25 cm) was measured from the medium fortified with 1 mg/mL indole-3-butyric acid (IBA) and 0.6 mg/mL indole-3-acetic acid (IAA), respectively. RP was successfully acclimated (85%) in vivo. Molecular analysis showed that the AP was true to the type of the MP. -Sabinene hydrate (26.8-57.7), 1,8-cineole (6.2-24.1), 4aα,7β,7aα-nepetalactone (4.1-12.3), and terpinene-4-ol (3.2-15.0) were the major essential oils compounds. The studied samples contained rosmarinic acid (2.55-5.97 mg/g DW), cichoric acid (1.68-12.7 mg/g DW), chlorogenic acid (1.91-64.21 mg/g DW), rutin (0.59-1.09 mg/g DW), apigenin (0.52-0.72 mg/g DW), betulinic acid (0.17-2.20 mg g DW), oleanolic acid (0.84-5.37 mg/g DW) and ursolic acid (3.46-15.70 mg/g DW). Acclimated plant exhibited the highest antioxidant activity (IC = 196.4 μg/mL), while the methanolic extract of MP displayed the highest antibacterial activity (MIC = 8 mg/mL) against .

SUPPLEMENTARY INFORMATION

The online version contains supplementary material available at 10.1007/s12298-024-01416-x.

摘要

未标注

首次引入了一种高效的体外培养方案，用于保护一种在伊朗中部发现的易危和濒危药用植物。将体外再生植株（RP）和驯化植株（AP）的生长、植物化学和生物学特性与母株（MP）进行了比较。此外，使用简单序列重复区间（ISSR）标记评估了AP的遗传稳定性。从添加3 mg/mL激动素（KIN）的培养基中获得的侧枝数量最多（4.25个），而在添加3 mg/mL噻二唑素（TDZ）和6-苄基腺嘌呤（BAP）的培养基上，侧枝长度最长（13.25 cm）。从含有3 mg/mL TDZ的培养基中获得的叶片数量最多（20.25片）和主枝长度最长（12.25 cm）。分别从添加1 mg/mL吲哚-3-丁酸（IBA）和0.6 mg/mL吲哚-3-乙酸（IAA）的培养基中测得的根数量最多（46.25条）和根长度最长（2.25 cm）。RP在体内成功驯化（85%）。分子分析表明，AP与MP类型一致。水合桧烯（26.8 - 57.7）、1,8-桉叶素（6.2 - 24.1）、4aα,7β,7aα-新薄荷醇（4.1 - 12.3）和萜品烯-4-醇（3.2 - 15.0）是主要的精油化合物。所研究的样品含有迷迭香酸（2.55 - 5.97 mg/g干重）、菊苣酸（1.68 - 12.7 mg/g干重）、绿原酸（1.91 - 64.21 mg/g干重）、芦丁（0.59 - 1.09 mg/g干重）、芹菜素（0.52 - 0.72 mg/g干重）、桦木酸（0.17 - 2.20 mg/g干重）、齐墩果酸（0.84 - 5.37 mg/g干重）和熊果酸（3.46 - 15.70 mg/g干重）。驯化植株表现出最高的抗氧化活性（IC = 196.4 μg/mL），而MP的甲醇提取物对[具体细菌名称未给出]表现出最高的抗菌活性（MIC = 8 mg/mL）。