Ye Danjuan, Duan Xiaoling, Guan Bin, Yuan Jia, Zhu Yan, Shi Jimin, Lu Qi, Xu Guoxiong
Department of Obstetrics and Gynaecology, Jinshan Hospital, Fudan University, Shanghai, P. R. China.
Research Center for Clinical Medicine, Jinshan Hospital, Fudan University, Shanghai, P. R. China.
J Int Med Res. 2024 Mar;52(3):3000605241233959. doi: 10.1177/03000605241233959.
Cervical cancer (CC) is one of the most common gynecologic malignancies worldwide. Although rapid improvements have been made regarding its prevention and treatment, little is known about disease pathogenesis and the clinical relevance of reliable biomarkers. The present study evaluated the expression of cystatin B (CSTB) as a potential biomarker of CC.
Tissue microarray analysis and immunohistochemical staining were performed to detect CSTB expression, while mRNA and protein expression levels of freshly isolated CC tissue were measured by quantitative real-time PCR and western blot, respectively. Bioinformatics were used to analyze the co-expression network and functional enrichments.
We observed high mRNA and protein expression levels in CC tissues, which was confirmed by tissue microarray in a comparison with paired adjacent non-cancerous cervical tissue samples. CSTB gene enrichments and associations with co-expressed genes were also observed. Further analysis showed that elevated CSTB expression was associated with pathological progress in CC.
Our data demonstrate that CSTB has the potential to be used as a tissue biomarker with clinical value in patients with CC, which may aid the development of intervention strategies.
宫颈癌（CC）是全球最常见的妇科恶性肿瘤之一。尽管在其预防和治疗方面已取得快速进展，但对疾病发病机制以及可靠生物标志物的临床相关性了解甚少。本研究评估了胱抑素B（CSTB）作为CC潜在生物标志物的表达情况。
进行组织微阵列分析和免疫组化染色以检测CSTB表达，同时分别通过定量实时PCR和蛋白质印迹法测量新鲜分离的CC组织的mRNA和蛋白质表达水平。利用生物信息学分析共表达网络和功能富集情况。
我们观察到CC组织中mRNA和蛋白质表达水平较高，与配对的相邻非癌宫颈组织样本相比，组织微阵列证实了这一点。还观察到CSTB基因富集以及与共表达基因的关联。进一步分析表明，CSTB表达升高与CC的病理进展相关。
我们的数据表明，CSTB有可能作为一种对CC患者具有临床价值的组织生物标志物，这可能有助于制定干预策略。
