Division of Applied Life Sciences (BK21), Gyeongsang National University, Jinju, 52828, Republic of Korea.
Department of Food Science & Technology and Institute of Agriculture & Life Science, Gyeongsang National University, Jinju, 52828, Republic of Korea.
Bioprocess Biosyst Eng. 2024 Mar;47(3):393-401. doi: 10.1007/s00449-024-02971-z. Epub 2024 Mar 4.
Calcium hypochlorite (Ca(ClO)), which can be stably stored in powder form for a long period, is widely used as a disinfectant in various fields. A new sterilization process was developed in the present study, where a microalgal medium was sterilized using 0.02% Ca(ClO), followed by complete neutralization of the Ca(ClO) within 8 h through catalytic reaction of an MnCl-NaEDTA complex and a synergistic effect of glucose. When comparing the growth of Chlorella vulgaris in the autoclaved medium, a 2.65 times greater maximum cell growth was observed in cells grown in the medium prepared by treatment of Ca(ClO). This result indicates that denaturation of the medium by heat can hinder the growth of some microorganisms. In the case of cultivation of Euglena gracilis, successful culture growth was achieved without growth inhibition or contamination on a medium prepared in the same manner.
次氯酸钙（Ca(ClO)）在粉末形式下可以稳定储存很长时间，被广泛用作各种领域的消毒剂。本研究开发了一种新的灭菌工艺，即用 0.02%的 Ca(ClO) 对微藻培养基进行灭菌，然后通过 MnCl-NaEDTA 配合物的催化反应和葡萄糖的协同作用在 8 小时内完全中和 Ca(ClO)。在比较经高压灭菌的培养基中普通小球藻的生长情况时，发现在用 Ca(ClO) 处理制备的培养基中培养的细胞的最大细胞生长速度快了 2.65 倍。这一结果表明，热变性的培养基可能会阻碍某些微生物的生长。在培养绿眼虫时，以同样的方式制备的培养基可以成功培养，没有生长抑制或污染。
