Department of Chemical and Biomolecular Engineering, KAIST, 291 Daehak-ro, Yuseong-gu, Daejeon 305-701, Republic of Korea.
Department of Chemical and Biomolecular Engineering, KAIST, 291 Daehak-ro, Yuseong-gu, Daejeon 305-701, Republic of Korea; Advanced Biomass R&D Center, KAIST, 291 Daehak-ro, Yuseong-gu, Daejeon 305-701, Republic of Korea.
Bioresour Technol. 2014 Nov;171:343-9. doi: 10.1016/j.biortech.2014.08.109. Epub 2014 Aug 30.
Mass cultivation of microalgae is necessary to achieve economically feasible production of microalgal biodiesel, but the high cost of nutrients is a major limitation. In this study, orange peel extract (OPE) was used as an inorganic and organic nutrient source for the cultivation of Chlorella vulgaris OW-01. Chemical composition analysis of the OPE indicated that it contains sufficient nutrients for mixotrophic cultivation of C. vulgaris OW-01. Analysis of biomass and FAME production showed that microalgae grown in OPE medium produced 3.4-times more biomass and 4.5-times more fatty acid methyl esters (FAMEs) than cells cultured in glucose-supplemented BG 11 medium (BG-G). These results suggest that growth of microalgae in an OPE-supplemented medium increases lipid production and that OPE has potential for use in the mass cultivation of microalgae.
大规模培养微藻是实现微藻生物柴油经济可行生产的必要条件，但营养物成本高是主要限制因素。在本研究中，橙皮提取物 (OPE) 被用作小球藻 OW-01 的混合营养培养的无机和有机营养源。OPE 的化学成分分析表明，它含有足够的营养物质用于小球藻 OW-01 的混合营养培养。生物质和 FAME 生产分析表明，在 OPE 培养基中生长的微藻产生的生物量比在补充葡萄糖的 BG 11 培养基 (BG-G) 中培养的细胞多 3.4 倍，脂肪酸甲酯 (FAMEs) 多 4.5 倍。这些结果表明，在 OPE 补充培养基中培养微藻可以增加油脂产量，并且 OPE 具有在微藻大规模培养中的应用潜力。
