Guangxi Engineering Research Center of Processing and Storage of Characteristic and Advantage Aquatic Products, Guangxi Academy of Fishery Sciences, Nanning, 530021, China.
Aquatic Technology Promotion Station in Jinchengjiang District, Hechi, 547000, China.
Arch Microbiol. 2024 Mar 4;206(4):139. doi: 10.1007/s00203-024-03853-4.
Salmonella exhibits a strong inducible acid tolerance response (ATR) under weak acid conditions, and can also induce high-risk strains that are highly toxic, acid resistant, and osmotic pressure resistant to aquatic products. However, the induction mechanism is not yet clear. Therefore, this study aims to simulate the slightly acidic, low-temperature, and high-protein environment during squid processing and storage. Through λRed gene knockout, exploring the effects of low-acid induction, long-term low-temperature storage, and two-component regulation on Salmonella ATR. In this study, we found the two-component system, PhoP/PhoQ and PmrA/PmrB in Salmonella regulates the amino acid metabolism system and improves bacterial acid tolerance by controlling arginine and lysine. Compared with the two indicators of total biogenic amine and diamine content, biogenic amine index and quality index were more suitable for evaluating the quality of aquatic products. The result showed that low-temperature treatment could inhibit Salmonella-induced ATR, which further explained the ATR mechanism from the amino acid metabolism.
沙门氏菌在弱酸条件下表现出强烈的诱导酸耐受反应（ATR），并且还可以诱导对水产品具有高毒性、耐酸和耐渗透压的高风险菌株。然而，其诱导机制尚不清楚。因此，本研究旨在模拟鱿鱼加工和储存过程中的微酸、低温和高蛋白环境。通过 λRed 基因敲除，探讨低酸诱导、长期低温储存和双组分调节对沙门氏菌 ATR 的影响。在这项研究中，我们发现沙门氏菌中的双组分系统 PhoP/PhoQ 和 PmrA/PmrB 通过控制精氨酸和赖氨酸来调节氨基酸代谢系统并提高细菌的酸耐受性。与总生物胺和二胺含量的两个指标相比，生物胺指数和质量指数更适合评估水产品的质量。结果表明，低温处理可以抑制沙门氏菌诱导的 ATR，这从氨基酸代谢角度进一步解释了 ATR 机制。
