College of Chemistry and Materials Science, Anhui Key Laboratory of Chem-Biosensing, Anhui Normal University, Wuhu, 241002, People's Republic of China.
Mikrochim Acta. 2024 Mar 4;191(4):176. doi: 10.1007/s00604-024-06254-y.
A sensitive electrochemical strategy for carcinoembryonic antigen 15-3 (CA15-3) detection is reported using CTAB-Co-MOFs@AuPt NPs as signal probes. The electrochemical strategy was designed as follows: First, the graphene aerogel@gold nanoparticles (GA@Au NPs) nanocomposites were employed to modify the sensing surface for promoting electron transfer rate and primary antibody (Ab) immobilization due to GA possesses a large specific surface area, eminent conductivity, and a 3D network structure. Cobalt metal-organic frameworks (CTAB-Co-MOFs) synthesized were then used as a carrier for AuPt NPs and secondary antibody (Ab) immobilization (notes: labelled-Ab). With sandwich immunoreaction, the labelled-Ab was captured on the surface of the GA@Au NPs nanocomposites. Finally, differential pulse voltammetry (DPV) was employed to register the electrochemical signal of the immunosensor at the potential of - 0.85 V (vs SCE) in phosphate buffer saline (PBS) containing 2.5 mM HO. It was verified that the electrochemical reduction signal from Co to Co was recorded. The AuPt NPs could catalyze the reaction of HO oxidizing Co to Co, resulting in the amplification of the electrochemical signal. Under the selected conditions, the immunosensor can detect CA15-3 in the range 10 µU/mL to 250 U/mL with a low detection limit of 1.1 µU/mL. In the designed strategy, the CTAB-Co-MOFs were not only employed as carriers for AuPt NPs, but also acted as signal probes. The CTAB-Co-MOFs were investigated including SEM, TEM, XPS, and XRD. The application ability of the immunosensor was evaluated using serum sample, demonstrating the immunosensor can be applied to clinic serum analysis.
一种基于 CTAB-Co-MOFs@AuPt NPs 的用于癌胚抗原 15-3（CA15-3）检测的灵敏电化学策略被报道。该电化学策略设计如下：首先，采用石墨烯气凝胶@gold 纳米粒子（GA@Au NPs）纳米复合材料修饰传感表面，以促进电子转移速率和一抗（Ab）固定，因为 GA 具有大的比表面积、优异的导电性和 3D 网络结构。然后，合成的钴金属-有机骨架（CTAB-Co-MOFs）被用作 AuPt NPs 和二抗（Ab）固定（注：标记-Ab）的载体。通过三明治免疫反应，标记-Ab 被捕获在 GA@Au NPs 纳米复合材料的表面上。最后，在含有 2.5mM HO 的磷酸盐缓冲盐水（PBS）中，在 -0.85V（相对于 SCE）的电位下，采用差分脉冲伏安法（DPV）记录免疫传感器的电化学信号。证明了从 Co 到 Co 的电化学还原信号被记录下来。AuPt NPs 可以催化 HO 将 Co 氧化为 Co 的反应，从而放大电化学信号。在选定的条件下，该免疫传感器可以在 10 µU/mL 至 250 U/mL 的范围内检测 CA15-3，检测限低至 1.1 µU/mL。在设计的策略中，CTAB-Co-MOFs 不仅用作 AuPt NPs 的载体，还用作信号探针。对 CTAB-Co-MOFs 进行了 SEM、TEM、XPS 和 XRD 研究。通过血清样本评估了免疫传感器的应用能力，表明该免疫传感器可用于临床血清分析。
