Key Laboratory for Green Processing of Chemical Engineering of Xinjiang Bingtuan, Engineering Research Center of Materials-Oriented Chemical Engineering of Xinjiang Production and Construction Corps, Key Laboratory of Materials-Oriented Chemical Engineering of Xinjiang Uygur Autonomous Region, School of Chemistry and Chemical Engineering, Shihezi University, Shihezi 832003, People's Republic of China.
Nanotechnology. 2020 May 1;31(18):185605. doi: 10.1088/1361-6528/ab70d3. Epub 2020 Jan 28.
Sandwich-type electrochemical immunosensor was one of the main methods for detecting carcinoembryonic antigen (CEA). In this work, using Ce-MoF as the skeleton precursor, hyaluronic acid (HA) was coated on the surface of Ce-metal organic framework (Ce-MoF), which loaded with silver nanoparticles (Ag NPs) and horseradish peroxidase (HRP) to catalyze HO and double amplified the current signal. Thus, a sensitive sandwich-type electrochemical immunosensor (Ce-MoF@ HA/Ag-HRP) was designed to detect carcinoembryonic antigen (CEA). The designed immunosensor used Au NPs to enhance the ability of attach more the first antibody (Ab). This was due to Au NPs had good electrical conductivity and biocompatibility to accelerate electron transfer on the surface of the electrode. HA was riched in -COOH, -OH and had excellent biocompatibility, which can carry more Ag NPs to catalyze HO. Finally, the prepared sandwich-type electrochemical immunosensor had excellent biocompatibility and great catalytic performance. The immunosensor can be tested within 30 min and the logarithm of the current signal and CEA concentration showed a broad linear response range of 1 pg ml-80 ng ml, and the detection limit of CEA was 0.2 pg ml. More importantly, the proposed immunosensor had good reproducibility, selectivity, stability and without matrix effect. This confirmed that the proposed immunosensor had broad prospects in early clinical trials.
三明治型电化学免疫传感器是检测癌胚抗原（CEA）的主要方法之一。在这项工作中，我们使用 Ce-MoF 作为骨架前体，在 Ce 金属有机骨架（Ce-MoF）表面涂覆透明质酸（HA），负载银纳米粒子（Ag NPs）和辣根过氧化物酶（HRP）以催化 HO 并双重放大电流信号。因此，设计了一种灵敏的三明治型电化学免疫传感器（Ce-MoF@HA/Ag-HRP）来检测癌胚抗原（CEA）。设计的免疫传感器使用 Au NPs 来增强附着更多第一抗体（Ab）的能力。这是因为 Au NPs 具有良好的导电性和生物相容性，可以加速电极表面的电子转移。HA 富含-COOH、-OH，具有出色的生物相容性，可携带更多的 Ag NPs 来催化 HO。最后，制备的三明治型电化学免疫传感器具有出色的生物相容性和优异的催化性能。该免疫传感器可以在 30 分钟内进行测试，电流信号的对数与 CEA 浓度呈宽线性响应范围为 1 pg ml-80 ng ml，CEA 的检测限为 0.2 pg ml。更重要的是，所提出的免疫传感器具有良好的重现性、选择性、稳定性，并且没有基质效应。这证实了所提出的免疫传感器在早期临床试验中具有广阔的前景。
