Men Xiao, Han Xionggao, Lee Se-Jeong, Oh Geon, Im Ji-Hyun, Bae Kwi Sik, Seong Geum-Su, La Im-Joung, Lee Do-Sang, Choi Sun-Il, Lee Ok-Hwan
Department of Food Biotechnology and Environmental Science, Kangwon National University, Chuncheon, 24341 Republic of Korea.
F&B Bio Co., Ltd, Cheonan, 31005 Korea.
Food Sci Biotechnol. 2023 Aug 18;33(5):1233-1243. doi: 10.1007/s10068-023-01407-w. eCollection 2024 Apr.
High doses or prolonged use of the exogenous synthetic glucocorticoid dexamethasone (Dex) can lead to muscle atrophy. In this study, the anti-atrophic effects of ginsenosides Rh1, Rg2, and Rg3 on Dex-induced C2C12 myotube atrophy were assessed by XTT, myotube diameter, fusion index, and western blot analysis. The XTT assay results showed that treatment with Rh1, Rg2, and Rg3 enhanced cell viability in Dex-injured C2C12 myotubes. Compared with the control group, the myotube diameter and fusion index were both reduced in Dex-treated cells, but treatment with Rh1, Rg2, and Rg3 increased these parameters. Furthermore, Rh1, Rg2, and Rg3 significantly downregulated the protein expression of FoxO3a, MuRF1, and Fbx32, while also upregulating mitochondrial biogenesis through the SIRT1/PGC-1α pathway. It also prevents myotube atrophy by regulating the IGF-1/Akt/ mTOR signaling pathway. These findings indicate that Rh1, Rg2, and Rg3 have great potential as useful agents for the prevention and treatment of muscle atrophy.
高剂量或长期使用外源性合成糖皮质激素地塞米松（Dex）可导致肌肉萎缩。在本研究中，通过XTT法、肌管直径、融合指数和蛋白质印迹分析评估了人参皂苷Rh1、Rg2和Rg3对Dex诱导的C2C12肌管萎缩的抗萎缩作用。XTT分析结果表明，Rh1、Rg2和Rg3处理可增强Dex损伤的C2C12肌管中的细胞活力。与对照组相比，Dex处理的细胞中肌管直径和融合指数均降低，但Rh1、Rg2和Rg3处理可增加这些参数。此外，Rh1、Rg2和Rg3显著下调FoxO3a、MuRF1和Fbx32的蛋白表达，同时还通过SIRT1/PGC-1α途径上调线粒体生物发生。它还通过调节IGF-1/Akt/mTOR信号通路来预防肌管萎缩。这些发现表明，Rh1、Rg2和Rg3作为预防和治疗肌肉萎缩的有效药物具有巨大潜力。
