Lin Deborah A, Abujamra Beatriz Abdo, Revah Stephanie, Nattkemper Leigh, Morrison Brian, Romanelli Paolo, Jozic Ivan
Dr. Phillip Frost Department of Dermatology and Cutaneous Surgery, Miller School of Medicine, University of Miami, Miami, Florida, USA.
JID Innov. 2024 Feb 1;4(2):100265. doi: 10.1016/j.xjidi.2024.100265. eCollection 2024 Mar.
We have previously identified that a structural membrane protein Caveolin-1 (Cav1) is involved in the regulation of aberrant keratinocyte proliferation and differentiation. The aim of this study was to elucidate the role of Cav1, Caveolin-2 (Cav2), and Cavin-1 in the pathogenesis of psoriasis vulgaris and between psoriasis subtypes. We utilized human biopsies from validated cases of psoriasis vulgaris (n = 21) at the University of Miami Hospital and compared the expression of Cav1, Cav2, and Cavin-1 by immunohistochemistry staining with that in normal healthy age-/sex-/location-matched skin (n = 15) and chronic spongiotic dermatitis skin samples (as control inflammatory skin condition) and quantified using QuPath. Distinct subtypes of psoriasis included guttate, inverse, nail, plaque, palmoplantar, and pustular. All biopsy samples exhibited a trend toward downregulation of Cav1, with nail, plaque, and palmoplantar psoriasis exhibiting the most pronounced effects. Only nail and pustular psoriasis samples exhibited significant downregulation of Cav2 and Cavin-1, suggesting Cav1 to be the main caveolar contributor to the pathogenesis of psoriasis. Together, these data support caveolae as pathophysiological targets in nail and pustular psoriasis, whereas Cav1 seems to be a general biomarker of multiple subtypes of psoriasis.
我们之前已经确定，一种结构性膜蛋白小窝蛋白-1（Cav1）参与了异常角质形成细胞增殖和分化的调控。本研究的目的是阐明Cav1、小窝蛋白-2（Cav2）和小窝蛋白结合蛋白-1（Cavin-1）在寻常型银屑病发病机制以及银屑病各亚型之间的作用。我们使用了迈阿密大学医院确诊的寻常型银屑病病例（n = 21）的人体活检组织，并通过免疫组织化学染色将Cav1、Cav2和Cavin-1的表达与年龄、性别、部位匹配的正常健康皮肤（n = 15）以及慢性海绵状皮炎皮肤样本（作为对照炎症性皮肤疾病）进行比较，并使用QuPath进行定量分析。银屑病的不同亚型包括点滴状、反向型、甲银屑病、斑块状、掌跖型和脓疱型。所有活检样本均呈现出Cav1下调的趋势，其中甲银屑病、斑块状银屑病和掌跖型银屑病的影响最为显著。只有甲银屑病和脓疱型银屑病样本表现出Cav2和Cavin-1的显著下调，这表明Cav1是银屑病发病机制中主要的小窝相关因素。总之，这些数据支持小窝作为甲银屑病和脓疱型银屑病的病理生理靶点，而Cav1似乎是多种银屑病亚型的通用生物标志物。
