Efficacy of commercially available entomopathogenic nematodes against insect pests of canola in Alberta, Canada.

Certain entomopathogenic nematodes (EPNs) in the families Steinernematidae and Heterorhabditidae are among the most studied biocontrol tools, some of which are commercially available against pest insects. Their use against foliar and subterranean insect pests is largely unexplored in the Canadian Prairies. We conducted a laboratory-based study to produce baseline information on the biocontrol potential of a few commercial EPN species. Percent mortality of flea beetles, diamondback moths (DBMs), lygus, cabbage root maggots, and black cutworms (BCWs) was assessed after 72 hours exposure to , , and at varying concentrations (25, 50, 100, and 200 infective juveniles (IJs) per larvae, pupae, or cm of soil surface). Irrespective of concentration level, and caused significant mortality in DBM and BCW larvae compared with , and were more efficient than in controlling root maggot larvae. caused zero mortality to root maggots at any concentration. Root maggot pupae were resistant to entry to EPN species tested, likely due to hard outer covering. Compared with root maggot pupae, a moderate level of mortality was observed in DBM pupae, suggesting differential ability of the tested EPNs in killing different life stages of certain pests. All nematode species tested caused low mortality (≤10%) in flea beetle adults. The findings of this investigation form fundamental data essential for carrying out field-based studies on canola and other related crops aimed at control and management of these pest species.