基于 g-CN-Au-鲁米诺纳米复合材料和 ZnCuS 纳米片的双增强夹心电化学发光适体传感器用于高灵敏度检测黏蛋白 1。
Double-enhanced sandwich electrochemiluminescence aptasensor based on g-CN-Au-luminol nanocomposites and ZnCuS nanosheets for highly sensitive detection of mucin 1.
机构信息
The Education Ministry Key Lab of Resource Chemistry, Joint International Research Laboratory of Resource Chemistry, Ministry of Education, Shanghai Frontiers Science Center of Biomimetic Catalysis, Shanghai Key Laboratory of Rare Earth Functional Materials, College of Chemistry and Materials Science, Shanghai Normal University, Shanghai 200234, China.
The Education Ministry Key Lab of Resource Chemistry, Joint International Research Laboratory of Resource Chemistry, Ministry of Education, Shanghai Frontiers Science Center of Biomimetic Catalysis, Shanghai Key Laboratory of Rare Earth Functional Materials, College of Chemistry and Materials Science, Shanghai Normal University, Shanghai 200234, China.
出版信息
Talanta. 2024 Jun 1;273:125867. doi: 10.1016/j.talanta.2024.125867. Epub 2024 Mar 1.
The traditional luminol electrochemiluminescence (ECL) sensing suffers from low signal response and instability issues. Here, an Au/ZnCuS double-enhanced g-CN-supported luminol ECL aptasensor is constructed for the sensitive detection of human mucin 1 (MUC1). In this platform, g-CN of a large specific surface area is beneficial to load more luminol illuminants. Au nanoparticles promote the decomposition of HO coreactants to generate more reactive oxygen (•OH and O) intermediates, while ZnCuS can immobilize the aptamer and simultaneously catalyze HO decomposition, realizing the double-wing signal amplification. Under optimal conditions, this sensor shows a good detection capability within 1.0 × 10-1.0 × 10 ng mL and a low detection limit of 5.0 × 10 ng mL, as well as ideal stability, selectivity, and reproducibility. This double-enhanced aptasensor highlights a new signal-enhancement approach for early biomarker detection.
传统的鲁米诺电化学发光（ECL）传感存在信号响应低和不稳定性问题。在这里，构建了一种 Au/ZnCuS 双重增强的 g-CN 负载鲁米诺 ECL 适体传感器，用于灵敏检测人粘蛋白 1（MUC1）。在该平台中，具有大比表面积的 g-CN 有利于负载更多的鲁米诺发光体。Au 纳米颗粒促进 HO 反应物的分解，产生更多的活性氧（•OH 和 O）中间体，而 ZnCuS 可以固定适体并同时催化 HO 分解，实现双翅信号放大。在最佳条件下，该传感器在 1.0×10-1.0×10ng mL 范围内具有良好的检测能力，检测限低至 5.0×10ng mL，具有理想的稳定性、选择性和重现性。这种双重增强的适体传感器为早期生物标志物检测提供了一种新的信号增强方法。
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