输卵管和子宫内膜上皮提高了体外生产的牛胚胎发育动力学。

Oviduct and endometrial epithelium improve in vitro produced bovine embryo developmental kinetics.

机构信息

Department of Animal Science, University of Tennessee Institute of Agriculture, University of Tennessee, Knoxville, Tennessee, USA.

出版信息

Reproduction. 2024 Apr 17;167(5). doi: 10.1530/REP-24-0008. Print 2024 May 1.

DOI:10.1530/REP-24-0008

PMID:38451876

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11056959/

Abstract

IN BRIEF

Standard in vitro produced (IVP) bovine embryo culture media limit embryonic development. Culturing IVP bovine embryos in standard IVP bovine embryo culture media conditioned with oviduct and/or endometrial cells improves blastocyst formation and reduces the time to formation.

ABSTRACT

In vitro embryo production in cattle greatly impacts blastomere biochemistry, embryo rate of development and pre- and post-transfer survival. In vivo, the bovine embryo migrates through the oviduct isthmus before entering the uterus on approximately day 4 of development where it remains unattached within the uterine lumen until day 20 of gestation. During this time, the embryo is sequentially exposed to oviduct followed by endometrial secretions that support embryonic development. Considering this, we tested the effect of culturing in vitro produced (IVP) bovine embryos sequentially in oviduct epithelial- (OEp; days 1-3) followed by endometrial epithelial- (EEp) or EEp and fibroblast cell (EEp/F; days 4-8)-conditioned media on embryonic development using a time-lapse monitoring system. Compared to control, culturing IVP embryos in EEp- or EEp/F-conditioned media without prior culture in OEp-conditioned media increased blastocyst formation (P < 0.05) and reduced the time to blastocyst formation (P < 0.05). Culturing IVP bovine embryos in OEp-conditioned media followed by EEp- or EEp/F-conditioned media, however, had the greatest impact on embryo developmental kinetics and increased morula and blastocyst formation (P < 0.05) and reduced time to formation (P < 0.05). Day 8 blastocyst cell numbers, diameter and quality were not significantly different, although, blastocyst quality scores were less (indicative of better quality) for all cell-conditioned media compared to control. In conclusion, IVP bovine embryo development may be improved using a sequential embryo culture system involving bovine oviduct followed by endometrial cell-conditioned media.

摘要

简介

标准的体外生产（IVP）牛胚胎培养液限制了胚胎的发育。在标准的 IVP 牛胚胎培养液中培养 IVP 牛胚胎，用输卵管和/或子宫内膜细胞条件化，可以提高囊胚形成率，并减少形成时间。

摘要

在牛中进行体外胚胎生产极大地影响了卵裂球的生化、胚胎发育速度和移植前及移植后的存活率。在体内，牛胚胎在发育的第 4 天左右通过输卵管峡部迁移，然后进入子宫，在那里直到妊娠第 20 天仍未附着在子宫腔中。在此期间，胚胎依次暴露于输卵管，然后暴露于支持胚胎发育的子宫内膜分泌物。考虑到这一点，我们测试了在体外生产（IVP）的牛胚胎在体外依次培养于输卵管上皮（OEp；第 1-3 天），然后培养于子宫内膜上皮（EEp）或 EEp 和成纤维细胞（EEp/F；第 4-8 天）条件培养液中的效果，使用时间 lapse 监测系统。与对照相比，在没有预先培养于 OEp 条件培养液的情况下，将 IVP 胚胎培养于 EEp 或 EEp/F 条件培养液中，提高了囊胚形成率（P<0.05），并缩短了囊胚形成时间（P<0.05）。然而，将 IVP 牛胚胎先培养于 OEp 条件培养液中，然后再培养于 EEp 或 EEp/F 条件培养液中，对胚胎发育动力学有最大的影响，提高了桑葚胚和囊胚的形成率（P<0.05），并缩短了形成时间（P<0.05）。第 8 天囊胚细胞数量、直径和质量没有显著差异，尽管与对照组相比，所有细胞条件培养液的囊胚质量评分都较低（表明质量较好）。总之，使用涉及牛输卵管的胚胎培养系统，然后用子宫内膜细胞条件培养液，可以改善 IVP 牛胚胎的发育。