State Key Laboratory of Transducer Technology, Aerospace Information Research Institute, Chinese Academy of Science, Beijing, 100190, China; School of Electronic, Electrical and Communication Engineering, University of Chinese Academy of Sciences, Beijing, 100149, China.
State Key Laboratory of Common Mechanism Research for Major Diseases, Institute of Basic Medical Sciences Chinese Academy of Medical Sciences, School of Basic Medicine Peking Union Medical College, Beijing, 100005, China.
Biosens Bioelectron. 2024 Jun 1;253:116168. doi: 10.1016/j.bios.2024.116168. Epub 2024 Mar 1.
Burst and local field potential (LFP) are fundamental components of brain activity, representing fast and slow rhythms, respectively. Understanding the intricate relationship between burst and LFP is crucial for deciphering the underlying mechanisms of brain dynamics. In this study, we fabricated high-performance microelectrode arrays (MEAs) using the SWCNTs/PEDOT:PSS nanocomposites, which exhibited favorable electrical properties (low impedance: 12.8 ± 2.44 kΩ) and minimal phase delay (-11.96 ± 1.64°). These MEAs enabled precise exploration of the burst-LFP interaction in cultured cortical networks. After a 14-day period of culture, we used the MEAs to monitor electrophysiological activities and revealed a time-locking relationship between burst and LFP, indicating the maturation of the neural network. To further investigate this relationship, we modulated burst firing patterns by treating the neural culture with increasing concentrations of glycine. The results indicated that glycine effectively altered burst firing patterns, with both duration and spike count increasing as the concentration rose. This was accompanied by an enhanced level of time-locking between burst and LFP but a decrease in synchrony among neurons. This study not only highlighted the pivotal role of SWCNTs/PEDOT:PSS-modified MEAs in elucidating the interaction between burst and LFP, bridging the gap between slow and fast brain rhythms in vitro but also provides valuable insights into the potential therapeutic strategies targeting neurological disorders associated with abnormal rhythm generation.
爆发和局部场电位 (LFP) 是大脑活动的基本组成部分，分别代表快速和慢速节律。理解爆发和 LFP 之间的复杂关系对于破译大脑动力学的潜在机制至关重要。在这项研究中，我们使用 SWCNTs/PEDOT:PSS 纳米复合材料制造了高性能微电极阵列 (MEA)，其表现出良好的电性能（低阻抗：12.8 ± 2.44 kΩ）和最小的相位延迟（-11.96 ± 1.64°）。这些 MEA 使我们能够精确地探索培养皮质网络中的爆发-LFP 相互作用。在培养 14 天后，我们使用 MEA 监测电生理活动，并揭示了爆发和 LFP 之间的时间锁定关系，表明神经网络的成熟。为了进一步研究这种关系，我们通过用不同浓度的甘氨酸处理神经培养物来调节爆发发射模式。结果表明，甘氨酸有效地改变了爆发发射模式，随着浓度的增加，持续时间和尖峰计数都增加。这伴随着爆发和 LFP 之间的时间锁定水平增强，但神经元之间的同步性降低。这项研究不仅强调了 SWCNTs/PEDOT:PSS 修饰的 MEA 在阐明爆发和 LFP 之间相互作用方面的关键作用，弥合了体外慢波和快波大脑节律之间的差距，而且为针对与异常节律产生相关的神经障碍的潜在治疗策略提供了有价值的见解。
