Parkinson Lily, Kuder Henrike, Kehl Alexandra, Koenig Amie, Good Jennifer, Hausmann Jennifer, Giger Urs
College of Veterinary Medicine, University of Georgia, Athens, GA 30602, USA,
Laboklin GmbH & Co. KG, 97688 Bad Kissingen, Germany.
J Zoo Wildl Med. 2024 Mar;55(1):143-154. doi: 10.1638/2023-0025.
Based upon previous clinical experience with domestic cats (), the ability to assess blood types and blood (in-)compatibilities of nondomestic felids, and adequately consider and plan for blood transfusions, may be important. Although nondomestic felids appear to have an blood group system similar to domestic cats, typing with point-of-care kits and by genotyping for domestic cats have not been reported. In this study, 162 blood samples from 18 different nondomestic felid species (cheetah [, = 42], lion [, = 33], tiger [, = 23], Canada lynx [, = 11], snow leopard [, = 10], puma [, = 7], clouded leopard [, = 6], serval [, = 5], jaguar [, = 5], fishing cat [, = 4], Pallas cat [, = 3], bobcat [, = 3], ocelot [, = 3], black footed cat [, = 2], leopard [, = 2], African wildcat [, = 1], caracal [, = 1], and sand cat [, = 1]) were blood typed by laboratory and point-of-care tests, genotyped for four known variants for type and type () phenotypes, and crossmatched with one another and domestic type cats. Traditional tube typing identified blood type ( = 106), type ( = 8), type ( = 43), and no discernible type ( = 4). Several discrepancies were found between point-of-care and traditional typing test results. None of the tested felids possessed the four CMAH variants responsible for type and () in domestic cats. Crossmatch incompatibilities (≥2+ agglutination) were identified within and between nondomestic felid species and beyond incompatibilities. Of 26 crossmatches performed between domestic cats and various nondomestic felids, only 7 (27%) were compatible. In conclusion, point-of-care typing kits and genotyping, successfully used in domestic cats, may not identify the correct blood type in nondomestic felids. Prior crossmatching is recommended to increase the likelihood of compatible transfusions between any nondomestic felids.
基于以往对家猫的临床经验，评估非家猫科动物的血型和血液（不）相容性，并充分考虑和规划输血，可能很重要。尽管非家猫科动物似乎具有与家猫相似的血型系统，但尚未有关于使用即时检测试剂盒对家猫进行血型鉴定和基因分型的报道。在本研究中，对来自18种不同非家猫科动物（猎豹[，n = 42]、狮子[，n = 33]、老虎[，n = 23]、加拿大猞猁[，n = 11]、雪豹[，n = 10]、美洲狮[，n = 7]、云豹[，n = 6]、薮猫[，n = 5]、美洲豹[，n = 5]、渔猫[，n = 4]、兔狲[，n = 3]、短尾猫[，n = 3]、豹猫[，n = 3]、黑足猫[，n = 2]、豹[，n = 2]、非洲野猫[，n = 1]、狞猫[，n = 1]和沙猫[，n = 1]）的162份血液样本进行了实验室和即时检测血型鉴定，对四种已知的A和B（）表型变体进行了基因分型，并相互之间以及与家猫A型进行了交叉配血。传统试管分型鉴定出A型（n = 106）、B型（n = 8）、AB型（n = 43）和无法识别的血型（n = 4）。在即时检测和传统分型检测结果之间发现了一些差异。在所检测的猫科动物中，没有一种拥有在家猫中导致A型和B型（）的四种CMAH变体。在非家猫科动物种内和种间以及超出种间的不相容性中都发现了交叉配血不相容性（≥2+凝集）。在26次家猫与各种非家猫科动物之间的交叉配血中，只有7次（27%）是相容的。总之，在家猫中成功使用的即时检测分型试剂盒和基因分型，可能无法识别非家猫科动物的正确血型。建议事先进行交叉配血，以增加任何非家猫科动物之间输血相容的可能性。
