Kehl Alexandra, Kuder Henrike, Parkinson Lily, Koenig Amie, Langbein-Detsch Ines, Mueller Elisabeth, Giger Urs
Laboklin GmbH & Co. KG, 97688 Bad Kissingen, Germany.
Comparative Experimental Pathology, School of Medicine, Technical University of Munich (TUM), 81675 Munich, Germany.
Animals (Basel). 2024 Aug 22;14(16):2442. doi: 10.3390/ani14162442.
Different blood group systems have been characterized in people and other mammals. In domestic cats, the blood group system plays the most important clinical role and has been investigated extensively-from the phenotype to the molecular genetics. In non-domestic felids, phenotypic blood typing has been performed by different methods to detect the antigens, but the four informative markers in domestic cats were not able to identify types and () in non-domestic cats. In this study, 138 blood samples from 15 non-domestic (wild) felid species were investigated by exonic sequencing and genotyping for putative variants causing type or () and correlation to the respective blood phenotype. A total of 58 variants were found, including 15 missense and 43 synonymous variants. One variant (c.635G>C) was concordant with blood type (and ) in cheetahs and type in cougars, compared to blood type in all other felid species (lion, tiger, Canada lynx, snow leopard, clouded leopard, serval, jaguar, fishing cat, Pallas cat, bobcat, black footed cat, leopard, and sand cat). Since cheetahs and cougars belong to the genera puma, it could not be determined if the common variant is either a marker for type (or ) or is just common in pumas.
不同的血型系统已在人类和其他哺乳动物中得到鉴定。在家猫中，血型系统发挥着最重要的临床作用，并且已经从表型到分子遗传学进行了广泛研究。在非家猫科动物中，已通过不同方法进行表型血型鉴定以检测抗原，但家猫中的四个信息性标记无法识别非家猫的血型类型和（）。在本研究中，通过外显子测序和基因分型对来自15种非家（野生）猫科动物的138份血液样本进行了研究，以寻找导致血型或（）的假定变体，并将其与各自的血型表型相关联。总共发现了58个变体，包括15个错义变体和43个同义变体。与所有其他猫科动物（狮子、老虎、加拿大猞猁、雪豹、云豹、薮猫、美洲虎、渔猫、兔狲、短尾猫、黑足猫、豹和沙猫）的血型相比，一个变体（c.635G>C）在猎豹和美洲狮中与血型（和）一致，在美洲狮中与血型一致。由于猎豹和美洲狮属于美洲狮属，因此无法确定该常见变体是血型（或）的标记还是仅在美洲狮属中常见。
