Department of Biomaterials Science, College of Dentistry, Dankook University, 119 Dandae-ro, Cheonan, Chungcheongnam-do 31116, Republic of Korea.
Institute of Tissue Regeneration Engineering (ITREN), Dankook University, 119 Dandae-ro, Cheonan, Chungcheongnam-do 31116, Republic of Korea.
ACS Biomater Sci Eng. 2024 Apr 8;10(4):2151-2164. doi: 10.1021/acsbiomaterials.3c01817. Epub 2024 Mar 7.
Poly(methyl methacrylate) (PMMA) is commonly used for dental dentures, but it has the drawback of promoting oral health risks due to oral bacterial adhesion. Recently, various nanoparticles have been incorporated into PMMA to tackle these issues. This study aims to investigate the mechanophysical and antimicrobial adhesive properties of a denture resin by incorporating of nanoclay into PMMA. Specimens were prepared by adding 0, 1, 2, and 4 wt % surface-modified nanoclay (Sigma) to self-polymerizing PMMA denture resin. These specimens were then evaluated using FTIR, TGA/DTG, and FE-SEM with EDS. Various mechanical and surface physical properties, including nanoindentation, were measured and compared with those of pure PMMA. Antiadhesion experiments were conducted by applying a (ATCC 11006) suspension to the surface of the specimens. The antiadhesion activity of was confirmed through a yeast-wall component (mannan) and mRNA-seq analysis. The bulk mechanical properties of nanoclay-PMMA composites were decreased compared to those of pure PMMA, while the flexural strength and modulus met the ISO 20795-1 requirement. However, there were no significant differences in the nanoindentation hardness and elastic modulus. The surface energy revealed a significant decrease at 4 wt % nanoclay-PMMA. The antiadhesion effect of was evident along with nanoclay content in the nanocomposites and confirmed by the reduced attachment of mannan on nanoclay-PMMA composites. mRNA-seq analysis supported overall transcriptome changes in altering attachment and metabolism behaviors on the surface. The nanoclay-PMMA materials showed a lower surface energy as the content increased, leading to an antiadhesion effect against . These findings indicate that incorporating nanoclay into PMMA surfaces could be a valuable strategy for preventing the fungal biofilm formation of denture base materials.
聚甲基丙烯酸甲酯（PMMA）常用于牙科义齿，但由于口腔细菌黏附，存在促进口腔健康风险的缺点。最近，各种纳米颗粒已被掺入 PMMA 中以解决这些问题。本研究旨在通过将纳米粘土掺入 PMMA 来研究义齿树脂的力学和抗菌黏附性能。通过向自聚合 PMMA 义齿树脂中添加 0、1、2 和 4wt%表面改性纳米粘土（Sigma）来制备样品。然后使用 FTIR、TGA/DTG 和 FE-SEM 与 EDS 对这些样品进行评估。测量了各种机械和表面物理性能，包括纳米压痕，并与纯 PMMA 进行了比较。通过将（ATCC 11006）悬浮液施加到样品表面来进行抗黏附实验。通过酵母细胞壁成分（甘露聚糖）和 mRNA-seq 分析证实了的抗黏附活性。与纯 PMMA 相比，纳米粘土-PMMA 复合材料的体积力学性能降低，而弯曲强度和模量符合 ISO 20795-1 要求。然而，纳米压痕硬度和弹性模量没有显著差异。表面能在添加 4wt%纳米粘土-PMMA 时显著降低。随着纳米复合材料中纳米粘土含量的增加，显示出明显的抗黏附效果，并且通过减少纳米粘土-PMMA 复合材料上甘露聚糖的附着得到证实。mRNA-seq 分析支持在改变表面附着和代谢行为方面的整体转录组变化。随着含量的增加，纳米粘土-PMMA 材料的表面能降低，从而对产生抗真菌生物膜的产生有抑制作用。这些发现表明，将纳米粘土掺入 PMMA 表面可能是防止义齿基材料真菌生物膜形成的一种有价值的策略。
