N-乙酰半胱氨酸和S-腺苷-L-蛋氨酸对赭曲霉毒素A诱导的大鼠肾毒性和免疫毒性的保护作用。
Protective effects of N-acetylcysteine and S-adenosyl-Lmethionine against nephrotoxicity and immunotoxicity induced by ochratoxin A in rats.
作者信息
Alkhattabi Nuha A, Khalifa Fares K, Doghaither Huda A Al, Al-Ghafari Ayat B, Tarbiah Nesrin Ibrahim, Sabban Aliaa
机构信息
Department of Biochemistry, Faculty of Science, King Abdulaziz University, Jeddah, Saudi Arabia.
Department of Biochemistry and Nutrition, Faculty of Women for Arts, Science, and Education, Ain Shams University, Cairo, Egypt.
出版信息
Int J Health Sci (Qassim). 2024 Mar-Apr;18(2):17-24.
OBJECTIVE
The present study was designed to investigate the nephroprotective and immunoprotective effects of S-adenosyl-L-methionine (SAMe) in comparison to N-acetylcysteine (NAC) against ochratoxin A (OTA) - intoxication.
METHODS
Forty-eight adult male Sprague-Dawley rats were categorized into four groups: Control; OTA intoxication (5 mg OTA/kg diet); OTA + NAC, rats received 200 mg NAC/day before feeding balanced diet contaminated with OTA; and (OTA + SAMe). Rats received 200 mg SAMe/day dissolved in distilled water orally just before feeding a balanced diet contaminated with OTA.
RESULTS
OTA administration altered serum kidney function biomarkers. These effects were pronouncedly alleviated by treatment with NAC. Results revealed a correlation between OTA-induced immunotoxicity and the reduced white blood cell (WBC) count. Treatments with SAMe significantly improved the WBCs count and hemoglobin concentration.
CONCLUSION
NAC and SAMe have a protective role against nephrotoxicity and immunotoxicity induced by continuous administration of OTA. NAC was more effective in reducing OTA nephrotoxicity, whereas SAMe was more potent than NAC in reducing OTA immunotoxicity.
目的
本研究旨在比较S-腺苷-L-蛋氨酸（SAMe）与N-乙酰半胱氨酸（NAC）对赭曲霉毒素A（OTA）中毒的肾保护和免疫保护作用。
方法
将48只成年雄性Sprague-Dawley大鼠分为四组：对照组；OTA中毒组（5 mg OTA/千克饮食）；OTA + NAC组，大鼠在喂食受OTA污染的平衡饮食前每天接受200 mg NAC；以及（OTA + SAMe）组。大鼠在喂食受OTA污染的平衡饮食前，每天口服溶解于蒸馏水中的200 mg SAMe。
结果
给予OTA改变了血清肾功能生物标志物。NAC治疗显著减轻了这些影响。结果显示OTA诱导的免疫毒性与白细胞（WBC）计数降低之间存在相关性。SAMe治疗显著改善了WBC计数和血红蛋白浓度。
结论
NAC和SAMe对连续给予OTA诱导的肾毒性和免疫毒性具有保护作用。NAC在降低OTA肾毒性方面更有效，而SAMe在降低OTA免疫毒性方面比NAC更有效。
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