Su Ran-Ran, Pan Bi-Qiong, Luo You-Xi, Zheng Xia-Lin, Lu Wen, Wang Xiao-Yun
Guangxi Key Laboratory of Agric-Environment and Agric-Products Safety, National Demonstration Center for Experimental Plant Science Education, College of Agriculture, Guangxi University, Nanning, China.
Front Bioeng Biotechnol. 2024 Feb 22;12:1340168. doi: 10.3389/fbioe.2024.1340168. eCollection 2024.
The intestinal bacteria of longhorn beetles would be ideal targets for pest control and lignocellulosic resources by destroying or exploiting their cellulose-degrading function. This article aims to investigate the diversity and community structure of intestinal bacteria the oligophagous longhorn beetle . Additionally, it seeks to identify the presence of lignocellulose-degrading bacteria in the gut, and explore their role in consuming host kapok trees . In this study, the bacterial community from was examined by Illumina sequencing of 16S ribosomal RNA (rRNA) targeting the V3 and V4 regions. A total of 563,201 valid sequences and 814 OTUs were obtained. The dominant phyla were Proteobacteria, and the dominant genera were and Lactococcus. The analysis of microbial diversity revealed a high bacterial diversity in the samples, with the gut bacteria playing a crucial role in the physiological activities of the host, particularly, 9 genera of intestinal bacteria with cellulose degradation function were found, highlighting their vital role in cellulose degradation. Five strains of cellulose-degrading bacteria, belonging to the genus were obtained from the intestinal tract of larvae using traditional isolation and culture techniques as well as 16S rDNA sequencing. Among these strains, A4 exhibited a cellulase activity of 94.42 ± 0.42 U/mL, while A5 displayed the highest filter paper enzyme activity of 127.46 ± 3.54 U/mL. These results offered valuable insights into potential targets for pest control through internal attack digestion and cellulose-degrading bacteria in longhorn beetles.
天牛的肠道细菌通过破坏或利用其纤维素降解功能，将成为害虫防治和木质纤维素资源利用的理想目标。本文旨在研究寡食性天牛肠道细菌的多样性和群落结构。此外，试图确定肠道中木质纤维素降解细菌的存在，并探讨它们在消耗寄主木棉树方面的作用。在本研究中，通过对16S核糖体RNA（rRNA）的V3和V4区域进行Illumina测序，检测了[具体来源未给出]的细菌群落。共获得563,201条有效序列和814个操作分类单元（OTU）。优势菌门为变形菌门，优势菌属为[具体未给出]和乳球菌属。微生物多样性分析显示样本中细菌多样性较高，肠道细菌在寄主的生理活动中起关键作用，特别是发现了9个具有纤维素降解功能的肠道细菌属，突出了它们在纤维素降解中的重要作用。利用传统分离培养技术以及16S rDNA测序，从[具体幼虫未给出]幼虫肠道中获得了5株属于[具体属未给出]的纤维素降解细菌。在这些菌株中，A4的纤维素酶活性为94.42±0.42 U/mL，而A5的滤纸酶活性最高，为127.46±3.54 U/mL。这些结果为通过内部攻击消化和天牛体内的纤维素降解细菌进行害虫防治的潜在目标提供了有价值的见解。
