载银纳米粒子水胶体薄膜促进大鼠糖尿病创面愈合。
silver nanoparticle-loaded hydrocolloid film for enhanced diabetic wound healing in rats.
机构信息
Department of Biochemistry and Biotechnology, University of Gujrat, Hafiz Hayat Campus Gujrat, Punjab, Pakistan.
Nawaz Sharif Medical College, University of Gujrat, Hafiz Hayat Campus Gujrat, Punjab, Pakistan.
出版信息
J Wound Care. 2024 Mar 1;33(Sup3a):xlviii-lx. doi: 10.12968/jowc.2024.33.Sup3a.xlviii.
OBJECTIVE
To investigate the role of silver nanoparticle-loaded carbopol gel for enhanced wound healing in a diabetic rat model. This research further aims to explore bioactive compounds derived from obtained from high altitude.
METHOD
Methanolic extracts of (MP), (MS) and (ML) were used to synthesise silver nanoparticles (AgNP). AgNP synthesis was confirmed by ultraviolet-visible (UV-Vis) spectroscopy, X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR) and scanning electron microscopy (SEM). The antioxidant activity was assessed by 2, 2-diphenyl-1-picrylhydrazyl (DDPH) assay. Antiglycation potential was determined by measuring the fluorescent advanced glycation end products. The bioactive compound identified in the methanolic (MPM) fraction through electrospray ionisation tandem mass spectrometric analysis (ESI-MS) was responsible for the highest antiglycation. The effects of MPM and MPM.AgNP-loaded Carbopol (Sanare Lab, India) on wound healing were compared in male, alloxan-induced, diabetic albino rats (200-250g), divided into control and treated groups. Effects on wound healing were assessed via histopathology.
RESULTS
UV-Vis and FTIR confirmed NP synthesis with peaks for flavonoids and polyphenols. SEM and XRD explored the cubical, 30-63nm crystalline NP. The maximum antioxidant and antiglycation potential was observed in order of; MP.AgNP>MS.AgNP>ML.AgNP. The highest antioxidant activity was observed by methanolic and aqueous MP.AgNPs (88.55% and 83.63%, respectively) at 2mg.ml, and (75.16% and 69.73%, respectively) at 1mg.ml, compared to ascorbic acid (acting as a positive control, 90.01%). MPM.AgNPs demonstrated the best antiglycation potential of 75.2% and 83.3% at 1mg.ml and 2mg.ml, respectively, comparable to positive control (rutin: 88.1%) at 14 days post-incubation. A similar trend was observed for antimicrobial activity against and with an inhibition zone of 21mm, 21.6mm and 24.6mm. Rosmarinic acid was the active compound present in , as identified by ESI-MS. MPM.AgNP-loaded Carbopol resulted in 100% wound closure compared with control at 20 days post-wounding. In the treatment group, re-epithelialisation was achieved by day 18, compared with 25 days for the positive control group.
CONCLUSION
MPM.AgNP-loaded Carbopol demonstrated safer and more effective biological properties, hence accelerating the diabetic excision wound healing process in alloxan-induced diabetic rats.
目的
研究载银纳米粒子的卡波姆凝胶在糖尿病大鼠模型中的增强伤口愈合作用。本研究旨在进一步探索从高海拔地区获得的生物活性化合物的作用。
方法
使用甲醇提取物（MP）、（MS）和（ML）合成银纳米粒子（AgNP）。AgNP 的合成通过紫外-可见（UV-Vis）光谱、X 射线衍射（XRD）、傅里叶变换红外光谱（FTIR）和扫描电子显微镜（SEM）进行确认。通过 2,2-二苯基-1-苦基肼（DDPH）测定法评估抗氧化活性。通过测量荧光高级糖基化终产物来确定抗糖化潜力。通过电喷雾串联质谱分析（ESI-MS）确定在 甲醇（MPM）部分中鉴定的生物活性化合物负责最高的抗糖化作用。比较了载有 MPM 和 MPM.AgNP 的卡波姆（Sanare Lab，印度）对雄性、链脲佐菌素诱导的糖尿病白化大鼠（200-250g）的伤口愈合作用，将其分为对照组和治疗组。通过组织病理学评估对伤口愈合的影响。
结果
UV-Vis 和 FTIR 证实了具有黄酮类和多酚类化合物特征的 NP 合成。SEM 和 XRD 研究了立方、30-63nm 结晶 NP。抗氧化和抗糖化潜力最大的顺序为；MP.AgNP>MS.AgNP>ML.AgNP。在 2mg.ml 和 1mg.ml 时，MP.AgNPs 的最高抗氧化活性分别为 88.55%和 83.63%（与阳性对照物抗坏血酸（90.01%）相比），而在 1mg.ml 和 69.73%（与阳性对照物抗坏血酸（90.01%）相比）时，MP.AgNPs 的最高抗氧化活性分别为 88.55%和 83.63%（与阳性对照物抗坏血酸（90.01%）相比）。在 1mg.ml 和 2mg.ml 时，MPM.AgNPs 表现出最佳的抗糖化潜力，分别为 75.2%和 83.3%，与 14 天孵育时的阳性对照物（芦丁：88.1%）相当。在抗菌活性方面，对 和 也观察到类似的趋势，抑菌圈为 21mm、21.6mm 和 24.6mm。通过 ESI-MS 鉴定出 中的活性化合物为迷迭香酸。与对照组相比，载有 MPM.AgNP 的卡波姆在 20 天伤口愈合后导致 100%的伤口闭合。在治疗组中，与阳性对照组的 25 天相比，在第 18 天就实现了再上皮化。
结论
载有 MPM.AgNP 的卡波姆凝胶表现出更安全、更有效的生物学特性，从而加速了糖尿病大鼠的糖尿病切除伤口愈合过程。
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