Anhui Medical University, Hefei, Anhui 230032, China; Department of Gynaecology, Yijishan Hospital of Wannan Medical College, Wuhu, Anhui 241000, China; Department of Gynaecology, The First People's Hospital of Wuhu, Wuhu, Anhui 241000, China.
Department of Gynaecology, The First People's Hospital of Wuhu, Wuhu, Anhui 241000, China.
Reprod Biol. 2024 Jun;24(2):100876. doi: 10.1016/j.repbio.2024.100876. Epub 2024 Mar 7.
Endometriosis is a chronic gynecological condition characterized by the presence of endometrial glands and stroma outside the uterine cavity., accounting for 7% of all female malignant tumors and 20%- 30% of malignant tumors of the female reproductive system. Multiple studies have shown that circular RNA (circRNA) has the potential to become a targeted target and marker for EM. However, the roles of circ_0001495 in EM are still unclear. Our research aims to reveal the molecular mechanism of circ_0001495 in EM. In this study, RT-PCR or western blot were conducted to determine mRNA and protein expression. cell viability, proliferation, migration, invasion, and apoptosis were assessed by CCK-8, EdU, wound healing, transwell, and flow cytometry analyses, respectively. Additionally, the targeting relationship between miR-34c-5p and circ_0001495 or E2F3 was confirmed through dual-luciferase reporter gene assay. We found significant overexpression of circ_0001495 in EM tissues and cells. Knockdown of circ_0001495 inhibited the proliferation, migration and invasion of ectopic endometrial stromal cells (EESCs) and increased cell apoptosis. Moreover, we found that circ_0001495 regulated E2F3 levels by interacting with miR-34c-5p in EESC. Furthermore, in vitro, miR-34c-5p inhibition or E2F3 overexpression could attenuate the effect of circ_0001495 silencing on EM progression. In addition, the vivo experiment demonstrated that inhibition of circ_0001495 could repress the development of endometriosis by regulating the miR-34c-5p/E2F3 axis. In conclusion, our study suggested that circ_0001495 promoted EM progression in vitro and in vivo through the miR-34c-5p/E2F3 axis, which might be a potential therapeutic target for EM.
子宫内膜异位症是一种慢性妇科疾病，其特征是子宫内膜腺体和基质出现在子宫腔外。它占所有女性恶性肿瘤的 7%，占女性生殖系统恶性肿瘤的 20%-30%。多项研究表明，环状 RNA (circRNA) 有可能成为子宫内膜异位症的靶向治疗靶点和标志物。然而，circ_0001495 在子宫内膜异位症中的作用尚不清楚。我们的研究旨在揭示 circ_0001495 在子宫内膜异位症中的分子机制。在这项研究中，通过 RT-PCR 或 Western blot 测定 mRNA 和蛋白表达。通过 CCK-8、EdU、划痕愈合、transwell 和流式细胞术分析分别评估细胞活力、增殖、迁移、侵袭和凋亡。此外，通过双荧光素酶报告基因检测证实了 miR-34c-5p 与 circ_0001495 或 E2F3 的靶向关系。我们发现 circ_0001495 在子宫内膜异位症组织和细胞中表达显著上调。circ_0001495 敲低抑制异位子宫内膜基质细胞 (EESCs) 的增殖、迁移和侵袭，并增加细胞凋亡。此外，我们发现 circ_0001495 通过与 miR-34c-5p 相互作用调节 EESC 中的 E2F3 水平。此外，在体外，miR-34c-5p 抑制或 E2F3 过表达可以减弱 circ_0001495 沉默对子宫内膜异位症进展的影响。此外，体内实验表明，通过调节 miR-34c-5p/E2F3 轴，抑制 circ_0001495 可以抑制子宫内膜异位症的发展。总之，我们的研究表明，circ_0001495 通过 miR-34c-5p/E2F3 轴在体内外促进子宫内膜异位症的进展，这可能是子宫内膜异位症的潜在治疗靶点。
