Faculty of Chemistry, Shahid Beheshti University, G. C., P.O. Box 198396-3113, Evin, Tehran, I.R. Iran.
Faculty of Chemistry, Shahid Beheshti University, G. C., P.O. Box 198396-3113, Evin, Tehran, I.R. Iran.
Food Chem. 2024 Jul 30;447:138848. doi: 10.1016/j.foodchem.2024.138848. Epub 2024 Feb 24.
This study presents a dispersive micro-solid phase extraction (D-μ-SPE) approach for extracting and determining of two organophosphorus pesticides (OPPs), including diazinon and chlorpyrifos as model analytes in various samples. For this purpose, we synthesized, characterized, and utilized magnetic multi-walled carbon nanotubes coated with poly 8-hydroxyquinoline (MWCNTs/FeO@PHQ) as a novel sorbent. The impact of various parameters, including sorbent type, sample pH, sample volume, sorbent amount, desorption solvent (type and volume), extraction time, and ionic strength on the extraction efficiency was investigated and optimized. Following the extraction, the desorbed pesticides in acetone were analyzed using gas chromatography with an FID detector. Under the optimized experimental conditions, the proposed method showed excellent linearity in the range of 3-1000 µg/L, low detection limit (0.9-1.5 µg/L), good relative recoveries (86-101.5 %), and high precision (RSD < 6.5 %). Finally, the applicability of this method was evaluated by analyzing the target OPPs in a variety of real samples, and obtained satisfactory results.
本研究提出了一种分散固相微萃取（D-μ-SPE）方法，用于提取和测定两种有机磷农药（OPPs），包括作为模型分析物的敌百虫和毒死蜱，这些分析物存在于各种样品中。为此，我们合成、表征并利用了多壁碳纳米管负载聚 8-羟基喹啉（MWCNTs/FeO@PHQ）作为一种新型吸附剂。考察并优化了各种参数（包括吸附剂类型、样品 pH 值、样品体积、吸附剂用量、解吸溶剂（类型和体积）、萃取时间和离子强度）对萃取效率的影响。萃取后，在 FID 检测器的气相色谱仪中用丙酮对洗脱的农药进行分析。在优化的实验条件下，该方法在 3-1000µg/L 的范围内表现出良好的线性，低检测限（0.9-1.5µg/L），良好的相对回收率（86-101.5%）和高精密度（RSD<6.5%）。最后，通过分析各种实际样品中的目标 OPPs 来评估该方法的适用性，得到了令人满意的结果。
