Dental Clinic, Department of Biomedical and Neuromotor Science (DIBINEM), Alma Mater Studiorum, University of Bologna, Italy.
Department of Surgery, Medicine, Dentistry and Morphological Sciences, University of Modena and Reggio Emilia, Italy.
Dent Mater. 2024 May;40(5):777-788. doi: 10.1016/j.dental.2024.03.003. Epub 2024 Mar 8.
To evaluate the effects of an ammonia-based and a water-based silver-containing solutions on bonding performance and matrix-metalloproteinases (MMPs) activity of a universal adhesive to dentin after 1 year of artificial aging.
Mid-coronal dentin surfaces of 60 sound human molars were exposed and the following groups were formed according to the surface pre-treatment and etching mode of the universal adhesive (Zipbond Universal, SDI) (n = 10): G1) Zipbond in the self-etch mode (ZSE); G2) Riva Star (SDI) applied before ZSE; G3) Riva Star Aqua (SDI) applied before ZSE; G4) Zipbond in the etch-and-rinse mode (ZER); G5) Riva Star applied before ZER; G6) Riva Star Aqua applied before ZER. The specimens were sectioned and subjected to microtensile bond strength (µTBS) test at baseline (T) and after 12 months (T) of artificial storage. Scanning electron microscope (SEM) and energy dispersive spectroscopy analysis (EDS) were also conducted. Three additional molars per group were processed for the in situ zymography analysis at T and T. Data were statistically analyzed (p < 0.05).
Dentin pre-treatments and aging decreased bonding values, regardless of the etching mode (p < 0.05). No differences in µTBS were observed between the two silver-containing solutions, both at T and T. Riva Star Aqua and etching significantly increased the MMPs activity, independent of the storage period (p < 0.05).
Dentin surface pre-treatment with silver-containing solutions negatively affects the bonding performances of resin composite restorations placed with a universal adhesive. However, the ammonia-based product Riva Star might show better stability in the long term, due to lower activation of MMPs.
评价氨基金属银溶液和水基金属银溶液对通用粘结剂与牙本质粘结性能和基质金属蛋白酶（MMPs）活性的影响，以及 1 年后人工老化的效果。
60 颗健康人磨牙的近中牙本质表面暴露，根据通用粘结剂（Zipbond Universal，SDI）的表面预处理和蚀刻模式，将牙齿分为以下几组（n=10）：G1）Zipbond 自酸蚀模式（ZSE）；G2）Riva Star（SDI）在 ZSE 之前应用；G3）Riva Star Aqua（SDI）在 ZSE 之前应用；G4）Zipbond 蚀刻-冲洗模式（ZER）；G5）Riva Star 在 ZER 之前应用；G6）Riva Star Aqua 在 ZER 之前应用。将标本切割并在基线（T）和 12 个月（T）的人工储存后进行微拉伸粘结强度（µTBS）测试。还进行了扫描电子显微镜（SEM）和能谱分析（EDS）。每组还额外处理了 3 颗磨牙，用于 T 和 T 时的原位酶谱分析。数据进行了统计学分析（p<0.05）。
无论蚀刻模式如何，牙本质预处理和老化都会降低粘结值（p<0.05）。两种含银溶液在 T 和 T 时的 µTBS 均无差异。Riva Star Aqua 和蚀刻显著增加了 MMPs 的活性，与储存期无关（p<0.05）。
含银溶液对牙本质表面的预处理会对使用通用粘结剂进行的树脂复合材料修复的粘结性能产生负面影响。然而，由于 MMPs 的激活较低，氨基金属银产品 Riva Star 可能在长期内表现出更好的稳定性。
