Inholz Katharina, Anderl Janet L, Klawitter Moritz, Goebel Heike, Maurits Elmer, Kirk Christopher J, Fan R Andrea, Basler Michael
Biotechnology Institute Thurgau (BITg) at the University of Konstanz, Kreuzlingen, Switzerland.
Division of Immunology, Department of Biology, University of Konstanz, Konstanz, Germany.
Eur J Immunol. 2024 Apr;54(4):e2350613. doi: 10.1002/eji.202350613. Epub 2024 Mar 8.
Immunoproteasomes are a special class of proteasomes, which can be induced with IFN-γ in an inflammatory environment. In recent years, it became evident that certain immune cell types constitutively express high levels of immunoproteasomes. However, information regarding the basal expression of proteolytically active immunoproteasome subunits in different types of immune cells is still rare. Hence, we quantified standard proteasome subunits (β1c, β2c, β5c) and immunoproteasome subunits (LMP2, MECL-1, LMP7) in the major murine (CD4 T cells, CD8 T cells, CD19 B cells, CD11c dendritic cells, CD49d natural killer cells, Ly-6G neutrophils) and human immune cell (CD4 T cells, CD8 T cells, CD19 B cells, CD1cCD141 myeloid dendritic cells, CD56 natural killer cells, granulocytes) subsets. The different human immune cell types were isolated from peripheral blood and the murine immune cell subsets from spleen. We found that proteasomes of most immune cell subsets mainly consist of immunoproteasome subunits. Our data will serve as a reference and guideline for immunoproteasome expression and imply a special role of immunoproteasomes in immune cells.
免疫蛋白酶体是一类特殊的蛋白酶体，在炎症环境中可被γ干扰素诱导产生。近年来，越来越明显的是，某些免疫细胞类型组成性地高表达免疫蛋白酶体。然而，关于不同类型免疫细胞中具有蛋白水解活性的免疫蛋白酶体亚基的基础表达信息仍然很少。因此，我们对主要的小鼠免疫细胞亚群（CD4 T细胞、CD8 T细胞、CD19 B细胞、CD11c树突状细胞、CD49d自然杀伤细胞、Ly-6G中性粒细胞）和人类免疫细胞亚群（CD4 T细胞、CD8 T细胞、CD19 B细胞、CD1cCD141髓样树突状细胞、CD56自然杀伤细胞、粒细胞）中的标准蛋白酶体亚基（β1c、β2c、β5c）和免疫蛋白酶体亚基（LMP2、MECL-1、LMP7）进行了定量。不同的人类免疫细胞类型从外周血中分离，小鼠免疫细胞亚群从脾脏中分离。我们发现，大多数免疫细胞亚群的蛋白酶体主要由免疫蛋白酶体亚基组成。我们的数据将作为免疫蛋白酶体表达的参考和指导，并暗示免疫蛋白酶体在免疫细胞中的特殊作用。
