Abdala-Torres Thais, Campi-Azevedo Ana Carolina, da Silva-Pereira Rosiane Aparecida, Dos Santos Luara Isabela, Henriques Priscilla Miranda, Costa-Rocha Ismael Artur, Otta Dayane Andriotti, Peruhype-Magalhães Vanessa, Teixeira-Carvalho Andréa, Araújo Márcio Sobreira Silva, Fernandes Eder Gatti, Sato Helena Keico, Fantinato Francieli Fontana Sutile Tardetti, Domingues Carla Magda Allan Santos, Kallás Esper Georges, Tomiyama Helena Tomoko Iwashita, Lemos Jandira Aparecida Campos, Coelho-Dos-Reis Jordana Grazziela, de Lima Sheila Maria Barbosa, Schwarcz Waleska Dias, de Souza Azevedo Adriana, Trindade Gisela Freitas, Ano Bom Ana Paula Dinis, da Silva Andrea Marques Vieira, Fernandes Camilla Bayma, Camacho Luiz Antônio Bastos, de Sousa Maia Maria de Lourdes, Martins-Filho Olindo Assis, do Antonelli Lis Ribeiro do Valle
Laboratório de Biologia e Imunologia de Doenças Infecciosas e Parasitárias, Instituto René Rachou, FIOCRUZ-Minas, Belo Horizonte, MG, Brazil.
Departamento de Bioquímica e Imunologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil.
NPJ Vaccines. 2024 Mar 8;9(1):54. doi: 10.1038/s41541-024-00836-w.
The re-emergence of yellow fever (YF) urged new mass vaccination campaigns and, in 2017, the World Health Organization approved the use of the fractional dose (FD) of the YF vaccine due to stock shortage. In an observational cross-sectional investigation, we have assessed viremia, antibodies, soluble mediators and effector and memory T and B-cells induced by primary vaccination of volunteers with FD and standard dose (SD). Similar viremia and levels of antibodies and soluble markers were induced early after immunization. However, a faster decrease in the latter was observed after SD. The FD led to a sustained expansion of helper T-cells and an increased expression of activation markers on T-cells early after vaccination. Although with different kinetics, expansion of plasma cells was induced upon SD and FD immunization. Integrative analysis reveals that FD induces a more complex network involving follicular helper T cells and B-cells than SD. Our findings substantiate that FD can replace SD inducing robust correlates of protective immune response against YF.
黄热病（YF）的再度出现促使开展新的大规模疫苗接种运动，2017年，由于库存短缺，世界卫生组织批准使用黄热病疫苗的分剂量（FD）。在一项观察性横断面调查中，我们评估了用FD和标准剂量（SD）对志愿者进行初次疫苗接种后诱导产生的病毒血症、抗体、可溶性介质以及效应和记忆T细胞及B细胞。免疫接种后早期诱导产生了相似的病毒血症以及抗体和可溶性标志物水平。然而，在接种SD后观察到后者下降得更快。FD导致辅助性T细胞持续扩增，且在接种疫苗后早期T细胞上激活标志物的表达增加。尽管动力学不同，但SD和FD免疫接种均诱导了浆细胞的扩增。综合分析表明，与SD相比，FD诱导了一个涉及滤泡辅助性T细胞和B细胞的更复杂网络。我们的研究结果证实，FD可替代SD诱导针对黄热病的强大保护性免疫反应相关指标。
