Department of Emergency Medicine, the First Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215006, China.
Department of Emergency Medicine, the First Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215006, China;
Allergol Immunopathol (Madr). 2024 Mar 1;52(2):60-67. doi: 10.15586/aei.v52i2.1068. eCollection 2024.
To explore the role of Y-box binding protein 1 (YBX-1) in the lipopolysaccharide (LPS)-stimulated inflammation and oxidative stress of BEAS-2B cell line and clarify the underlying mechanism.
LPS-stimulated BEAS-2B cells were used as a cell model of sepsis-stimulated acute lung injury (ALI). Immunoblot and quantitative polymerase chain reaction assays were used to detect the expression of YBX-1 in LPS-stimulated BEAS-2B cells. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide, TdT-mediated dUTP nick end labeling, and immunoblot assays were conducted to determine the effects of YBX-1 on cell survival. JC-1 staining and adenosine triphosphate production were used to detect the effects of YBX-1 on mitochondrial function. Immunostaining and enzyme-linked immunosorbent serologic assay were performed to examine the effects of YBX-1 on the inflammation and oxidative stress of cells. Immunoblot assay was conducted to confirm the mechanism.
YBX-1 was lowly expressed in LPS-stimulated BEAS-2B cells and enhanced the survival of LPS-stimulated lung epithelial cells. In addition, YBX-1 improved mitochondrial function of LPS-stimulated BEAS-2B cells. YBX-1 inhibited the inflammation and oxidative stress of LPS-stimulated BEAS-2B cells. Mechanically, YBX-1 inhibited mitogen-activated protein kinase (MAPK) axis, thereby alleviating sepsis-stimulated ALI.
YBX-1 alleviated inflammation and oxidative stress of LPS-stimulated BEAS-2B cells via MAPK axis.
探讨 Y 盒结合蛋白 1（YBX-1）在脂多糖（LPS）刺激的 BEAS-2B 细胞系炎症和氧化应激中的作用，并阐明其潜在机制。
采用 LPS 刺激的 BEAS-2B 细胞作为脓毒症刺激的急性肺损伤（ALI）的细胞模型。采用免疫印迹和定量聚合酶链反应检测 LPS 刺激的 BEAS-2B 细胞中 YBX-1 的表达。采用 3-(4,5-二甲基噻唑-2-基)-2,5-二苯基-2H-四唑溴盐（MTT）、末端转移酶介导的 dUTP 缺口末端标记（TUNEL）和免疫印迹检测 YBX-1 对细胞存活的影响。JC-1 染色和三磷酸腺苷（ATP）产生用于检测 YBX-1 对线粒体功能的影响。免疫染色和酶联免疫吸附试验（ELISA）用于检测 YBX-1 对细胞炎症和氧化应激的影响。免疫印迹用于验证机制。
YBX-1 在 LPS 刺激的 BEAS-2B 细胞中低表达，并增强 LPS 刺激的肺上皮细胞的存活。此外，YBX-1 改善了 LPS 刺激的 BEAS-2B 细胞的线粒体功能。YBX-1 抑制了 LPS 刺激的 BEAS-2B 细胞的炎症和氧化应激。机制上，YBX-1 抑制丝裂原活化蛋白激酶（MAPK）轴，从而减轻脓毒症引起的 ALI。
YBX-1 通过 MAPK 轴缓解 LPS 刺激的 BEAS-2B 细胞的炎症和氧化应激。
