利用 CRISPR/Cas9 技术生成两个 Bax 和 Bak1 双基因敲除的人诱导多能干细胞系。
Generation of two human induced pluripotent stem cell lines with BAX and BAK1 double knock-out using CRISPR/Cas9.
机构信息
Berlin Institute of Health at Charité - Universitätsmedizin Berlin, Core Unit pluripotent Stem Cells and Organoids, 13353 Berlin, Germany.
Charité - Universitätsmedizin Berlin, Dept of Neurology with Experimental Neurology, Charitéplatz 1, 10117 Berlin, Germany; Charité - Universitätsmedizin Berlin, Center for Stroke Research Berlin, Charitéplatz 1, 10117 Berlin, Germany.
出版信息
Stem Cell Res. 2024 Apr;76:103377. doi: 10.1016/j.scr.2024.103377. Epub 2024 Mar 5.
Bcl-2-associated X protein (BAX) and Blc-2 homologous antagonist killer 1 (BAK) are two pro-apoptotic members of BCL2 family. Here, two BAX/BAK double knock-out human induced pluripotent stem cell lines (iPSC) we generated using CRISPR-Cas9 to generate apoptosis incompetent cell lines. The resulting cell lines were karyotypically normal, had typical morphology and expressed typical markers for the undifferentiated state.
Bcl-2 相关 X 蛋白 (BAX) 和 Bcl-2 同源拮抗剂杀伤 1 (BAK) 是 BCL2 家族的两个促凋亡成员。在这里，我们使用 CRISPR-Cas9 生成了两个 BAX/BAK 双敲除人诱导多能干细胞系 (iPSC)，以生成凋亡无能的细胞系。所得细胞系的染色体核型正常，形态典型，并表达未分化状态的典型标志物。
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