Key Laboratory for Analytical Science of Food Safety and Biology (MOE & Fujian Province), Department of Chemistry, Fuzhou University, Fuzhou, 350108, PR China; Chongqing Key Laboratory of Kinase Modulators as Innovative Medicine, College of Pharmacy (International Academy of Targeted Therapeutics and Innovation), Chongqing University of Arts and Sciences, Chongqing, 402160, PR China.
Chongqing Key Laboratory of Kinase Modulators as Innovative Medicine, College of Pharmacy (International Academy of Targeted Therapeutics and Innovation), Chongqing University of Arts and Sciences, Chongqing, 402160, PR China.
Talanta. 2024 Jun 1;273:125863. doi: 10.1016/j.talanta.2024.125863. Epub 2024 Mar 6.
The development of rapid screening sensing platforms to improve pre-screening mechanisms in community healthcare is necessary to meet the significant need for portable testing in biomarker diagnostics. Here, we designed a portable smartphone-based photoelectrochemical (PEC) immunoassay for carcinoembryonic antigen (CEA) detection using Cu-doped ultrathin porous BiWO (CuBWO) nanosheets as the photoactive material. The CuBWO nanosheets exhibit a fast photocurrent response and excellent electrical transmission rate under UV light due to their surface plasmon resonance effect (SPR). The method uses glucose oxidase-labeled secondary antibody as a signal indicator for sandwich-type immune conjugation. In the presence of the target CEA, the electrons and holes generated at the surface of the photo-excited ultrathin porous CuBWO were rapidly consumed by the production of HO from glucose oxidase oxidizing glucose, resulting in a weakened photocurrent signal. The photocurrent intensity increased logarithmically and linearly with increasing CEA concentration (0.02-50 ng mL), with a detection limit of 15.0 pg mL (S/N = 3). The system provides a broader idea for inferring the electron-hole transport mechanism in ultrathin porous nanosheet layer materials and developing efficient PEC sensors.
为了满足生物标志物诊断中对便携式检测的巨大需求，有必要开发用于改善社区医疗中预筛选机制的快速筛选传感平台。在这里，我们设计了一种基于智能手机的光电化学（PEC）免疫分析方法，用于检测癌胚抗原（CEA），该方法使用掺铜的超薄多孔 BiWO（CuBWO）纳米片作为光活性材料。由于表面等离子体共振效应（SPR），CuBWO 纳米片在紫外光下表现出快速光电流响应和优异的电传输率。该方法使用葡萄糖氧化酶标记的二级抗体作为信号指示剂，用于夹心型免疫结合。在存在靶标 CEA 的情况下，在光激发超薄多孔 CuBWO 表面产生的电子和空穴被葡萄糖氧化酶氧化葡萄糖产生的 HO 迅速消耗，导致光电流信号减弱。光电流强度与 CEA 浓度（0.02-50ng mL）呈对数和线性增加，检测限为 15.0pg mL（S/N=3）。该系统为推断超薄多孔纳米片层材料中的电子-空穴输运机制和开发高效的 PEC 传感器提供了更广泛的思路。
