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从 opposita 叶下珠中提取的多糖的结构表征和免疫调节活性。

Structural characterization and immunomodulatory activity of a polysaccharide from Dioscotea opposita.

机构信息

College of Pharmacy, Hebei University of Chinese Medicine, 3 Xingyuan Road, Shijiazhuang 050200, China; Traditional Chinese Medicine Processing Technology Innovation Center of Hebei Province, 3 Xingyuan Road, Shijiazhuang 050200, China.

College of Chemistry and Pharmaceutical Engineering, Hebei University of Science and Technology, 26 Yuxiang Street, Shijiazhuang 050018, China.

出版信息

Int J Biol Macromol. 2024 Apr;265(Pt 1):130734. doi: 10.1016/j.ijbiomac.2024.130734. Epub 2024 Mar 8.

Abstract

The purified polysaccharides fraction, DOP-2, was prepared from Dioscorea opposita Thunb (D. opposita). This study combined in vitro and in vivo experiments to comprehensively investigate the index changes in RAW264.7 cells and immunocompromised mice under DOP-2 intervention, aiming to elucidate the potential mechanisms of immunomodulatory effects of DOP-2. DOP-2 (10 ∼ 500 μg/mL) significantly elevated the levels of NO, interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) factors secreted by RAW264.7 cells, and restored the body weight of immunosuppressed mice and improve the degree of injury to the immune organ index, resulting in significant immunomodulatory effects. Notably, DOP-2 promoted the production of short-chain fatty acids (SCFAs) in immunosuppressed mice and modulated the composition of their gut microflora. These findings highlight the potential benefits of DOP-2 therapy in improving immune function and gut health, and will provide a theoretical basis for the application of D. opposita polysaccharides as an immunomodulatory adjuvant.

摘要

从薯蓣科植物(Dioscorea opposita)中提取得到的纯化多糖 DOP-2。本研究通过体外和体内实验相结合,全面研究了 DOP-2 干预下 RAW264.7 细胞和免疫功能低下小鼠的各项指标变化,旨在阐明 DOP-2 免疫调节作用的潜在机制。DOP-2(10~500μg/mL)可显著提高 RAW264.7 细胞分泌的 NO、白细胞介素-6(IL-6)和肿瘤坏死因子-α(TNF-α)等因子的水平,并恢复免疫抑制小鼠的体重,改善免疫器官指数的损伤程度,具有显著的免疫调节作用。值得注意的是,DOP-2 可促进免疫抑制小鼠短链脂肪酸(SCFAs)的生成,并调节其肠道微生物群落组成。这些发现强调了 DOP-2 治疗在改善免疫功能和肠道健康方面的潜在益处,并将为薯蓣多糖作为免疫调节剂的应用提供理论依据。

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