Zhang Chuanxiang, Ding Jie, Bian Zhiying, Liu Xin, Wang Di, Cao Guiyun, Zhu Lihao, Zhang Jin, Liu Qian, Liu Yuhong
School of Pharmaceutical Sciences, Shandong University of Traditional Chinese Medicine, Jinan 250000, China.
Shandong Hongjitang Pharmaceutical Group Company, Ltd, Jinan 250109, China.
Int J Biol Macromol. 2024 Sep 17;280(Pt 1):135767. doi: 10.1016/j.ijbiomac.2024.135767.
To establish the quality control method of Dioscorea opposita Thunb., the multi-level fingerprinting of polysaccharides was established and the relationship between fingerprint and immune activity was analyzed. The two molecular weight segments Mw1 (1.38 × 10-1.63 × 10 Da) and Mw2 (3.27 × 10-4.37 × 10 Da), thirteen infrared absorption peaks (3399.26 cm, 2929.32 cm, 1631.78 cm, 1400.39 cm, 1351.80 cm, 1123.58 cm, 1024.76 cm, 931.53 cm, 854.76 cm, 760.43 cm, 708.14 cm, 616.47 cm, and 526.78 cm), and four monosaccharides (Man, Rha, GalA, and Glc) were used to evaluate the quality of Dioscorea opposita Thunb. The molecular weight fragments of Mw1, FT-IR absorption peaks of 1631.78 cm, and two monosaccharides (Man and Glc) would be used to identify Dioscorea opposita Thunb. polysaccharide (DOP) from different origins. The relationship of spectrum-effect showed that polysaccharides with features such as higher Mw1, a lower peak height of 1631.78 cm, higher content of Man, and lower content of Glc exerted stronger immune activity. In conclusion, this study established a polysaccharide-based quality evaluation method for Dioscorea opposita Thunb. and explored the relationship between polysaccharide fingerprints and in vitro immune activity, which provided a basis for further research on Dioscorea opposita Thunb.
为建立山药的质量控制方法，建立了多糖的多层次指纹图谱，并分析了指纹图谱与免疫活性之间的关系。利用两个分子量片段Mw1（1.38×10 - 1.63×10 Da）和Mw2（3.27×10 - 4.37×10 Da）、13个红外吸收峰（3399.26 cm、2929.32 cm、1631.78 cm、1400.39 cm、1351.80 cm、1123.58 cm、1024.76 cm、931.53 cm、854.76 cm、760.43 cm、708.14 cm、616.47 cm和526.78 cm）以及四种单糖（甘露糖、鼠李糖、半乳糖醛酸和葡萄糖）来评价山药的质量。Mw1的分子量片段、1631.78 cm的傅里叶变换红外吸收峰以及两种单糖（甘露糖和葡萄糖）将用于鉴别不同来源的山药多糖（DOP）。谱效关系表明，具有较高Mw1、较低的1631.78 cm峰高、较高的甘露糖含量和较低的葡萄糖含量等特征的多糖具有较强的免疫活性。综上所述，本研究建立了基于多糖的山药质量评价方法，探索了多糖指纹图谱与体外免疫活性之间的关系，为山药的进一步研究提供了依据。
