Sun Lili, Yeerkenbieke Buerlan, Yuemaierabola Anwaier, Liu Fuzhong, Yeerxiati Dilinaer, Dong Xiaogang, Guo Wenjia
Department of Cancer Research Institute, Affiliated Cancer Hospital of Xinjiang Medical University Urumqi 830011, Xinjiang, China.
Department of Hepatobiliary and Pancreatic Surgery, Affiliated Cancer Hospital of Xinjiang Medical University Urumqi 830011, Xinjiang, China.
Am J Transl Res. 2024 Feb 15;16(2):415-431. doi: 10.62347/MYMM7384. eCollection 2024.
Primary hepatocellular carcinoma (HCC) affects people all over the world. Circular RNAs are involved in the growth and development of several malignancies and regulate a number of biological processes. However, the roles of has-circ-0009158 in HCC remain unknown. This study explored the expression and associated miRNA-mRNA network of has-circ-0009158 in HCC. Quantitative real-time polymerase chain reaction was used to measure the expression of hsa-circ-0009158 in the HCC tissues of 143 patients and four human HCC cell lines. Then, the potential relationship of hsa-circ-0009158 expression with clinical characteristics and prognosis of patients was analyzed using the GO and KEGG databases. Correlated miRNA-mRNA networks were forecasted using the TCGA database and Cytoscape software. The hsa-circ-0009158 expression was significantly upregulated in HCC tissues and cell lines (P<0.001). The multivariate Cox analysis revealed that HCC patients were associated with high hsa-circ-0009158 expression. The bioinformatics analysis screened 1 miRNA, and 248 mRNAs associated with the circRNA in HCC. A pathway analysis suggested that the differentially expressed genes (DEGs) may be linked to the development and growth of HCC tumors. Ten hub genes (MELK, NCAPG, BUB1B, BIRC5, CDCA8, CENPF, BUB1, CDK1, TTK, TPX2) were identified from the PPI network based on the 248 genes. Additionally, the 10 hub genes that were verified had an association between high expression levels and low overall survival rates. As a result, the high expression of hsa-circ-0009158 was found to be a separate risk factor for recurrence and a poor prognosis in HCC patients.
原发性肝细胞癌（HCC）影响着世界各地的人们。环状RNA参与多种恶性肿瘤的生长和发展，并调节许多生物学过程。然而，has-circ-0009158在HCC中的作用仍不清楚。本研究探讨了has-circ-0009158在HCC中的表达及相关的miRNA-mRNA网络。采用定量实时聚合酶链反应检测143例患者的HCC组织和4种人HCC细胞系中hsa-circ-0009158的表达。然后，利用GO和KEGG数据库分析hsa-circ-0009158表达与患者临床特征及预后的潜在关系。使用TCGA数据库和Cytoscape软件预测相关的miRNA-mRNA网络。hsa-circ-0009158在HCC组织和细胞系中表达显著上调（P<0.001）。多因素Cox分析显示，HCC患者与hsa-circ-0009158高表达有关。生物信息学分析筛选出1个与HCC中circRNA相关的miRNA和248个mRNA。通路分析表明，差异表达基因（DEG）可能与HCC肿瘤的发生和生长有关。基于这248个基因从PPI网络中鉴定出10个枢纽基因（MELK、NCAPG、BUB1B、BIRC5、CDCA8、CENPF、BUB1、CDK1、TTK、TPX2）。此外，经验证的10个枢纽基因的高表达水平与低总生存率之间存在关联。结果发现，hsa-circ-0009158的高表达是HCC患者复发和预后不良的独立危险因素。
