Pak So-Won, Lee Se-Jin, Kim Woong-Il, Yang Yea-Gin, Cho Young-Kwon, Kim Joong-Sun, Kim Tae-Won, Ko Je-Won, Kim Jong-Choon, Kim Sung-Hwan, Shin In-Sik
College of Veterinary Medicine and BK21 FOUR Program, Chonnam National University, Buk-gu, Gwangju, Republic of Korea.
College of Health Sciences, Cheongju University, Cheongju-si, Chungbuk, Republic of Korea.
Vet Med (Praha). 2024 Jan 24;69(1):8-17. doi: 10.17221/77/2023-VETMED. eCollection 2024 Jan.
Asian sand dust (ASD), also called China dust or yellow dust, mainly occurs in East Asia during spring and autumn. Because ASD enters the body mainly through the respiratory system, it can cause respiratory disorders or worsen underlying diseases. Because of this, it has become an important health concern that threatens the well-being of humans and animals. In this study, we investigated the effects of 15 and 30 mg/kg of Pycnogenol (PYC15 and 30 groups), a pine bark extract, on ASD-induced pulmonary inflammation in mice. We evaluated the inflammatory cell counts, inflammatory cytokines, and matrix-metalloproteinase (MMP)-9 expression in animal models. PYC administration significantly decreased inflammatory cell infiltration into lung tissue; this was accompanied by a reduction in the levels of proinflammatory mediators including interleukin (IL)-1β ( 0.01), IL-6 ( 0.01) and tumour necrosis factor-α ( 0.01) in bronchoalveolar lavage fluids of ASD-exposed mice (ASD group). Histological analysis revealed that PYC suppressed ASD-induced pulmonary inflammation. Moreover, PYC suppressed the levels of matrix-metalloproteinase (MMP)-9 in the lung tissue of ASD-exposed mice, indicating that PYC reduced ASD-induced pulmonary inflammation by suppressing MMP-9. Together, these results indicate that PYC as the potential to treat ASD-driven pulmonary inflammation.
亚洲沙尘（ASD），也被称为中国沙尘或黄沙，主要在春秋季出现在东亚地区。由于亚洲沙尘主要通过呼吸系统进入人体，它会引发呼吸系统疾病或加重潜在疾病。因此，它已成为威胁人类和动物健康的一个重要问题。在本研究中，我们调查了15毫克/千克和30毫克/千克的碧萝芷（PYC15和30组，一种松树皮提取物）对亚洲沙尘诱导的小鼠肺部炎症的影响。我们评估了动物模型中的炎症细胞计数、炎症细胞因子和基质金属蛋白酶（MMP）-9的表达。给予碧萝芷显著减少了炎症细胞向肺组织的浸润；这伴随着暴露于亚洲沙尘的小鼠（ASD组）支气管肺泡灌洗液中促炎介质水平的降低，包括白细胞介素（IL）-1β（P<0.01）、IL-6（P<0.01）和肿瘤坏死因子-α（P<0.01）。组织学分析显示碧萝芷抑制了亚洲沙尘诱导的肺部炎症。此外，碧萝芷抑制了暴露于亚洲沙尘的小鼠肺组织中基质金属蛋白酶（MMP）-9的水平，表明碧萝芷通过抑制MMP-9减轻了亚洲沙尘诱导的肺部炎症。总之，这些结果表明碧萝芷具有治疗亚洲沙尘引发的肺部炎症的潜力。
