大鼠肝脏胱硫醚-γ-裂解酶催化S-（羧甲基硫基）半胱氨酸裂解的机制
Mechanism of S-(carboxymethylthio)cysteine cleavage by rat liver cystathionine-gamma-lyase.
作者信息
Yao K, Kinuta M, Watanabe Y
出版信息
Physiol Chem Phys. 1978;10(6):569-73.
PMID:38467
Abstract
Rat liver cystathionine-gamma-lyase [L-cystathionine cysteinelyase (deaminating), EC 4.4.1.1] catalyzes the formation of pyruvic acid, ammonia, and carboxymethylhydrodisulfide from S-(carboxymethylthio)cysteine (CMTC). As judged by pyruvic acid production, the optimal pH is 8.3 in tris-HCl buffer and the Km is 2.9 mM. A possible mechanism of CMTC cleavage by cystathionase is proposed.
摘要
大鼠肝脏胱硫醚-γ-裂解酶[S-（羧甲基硫基）半胱氨酸裂解酶（脱氨基），EC 4.4.1.1]催化由S-（羧甲基硫基）半胱氨酸（CMTC）生成丙酮酸、氨和羧甲基氢二硫化物。以丙酮酸生成量判断，在三羟甲基氨基甲烷盐酸盐缓冲液中，最适pH为8.3，米氏常数为2.9 mM。本文提出了胱硫醚酶裂解CMTC的一种可能机制。
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