College of Grassland Agriculture, Northwest A&F University, Yangling 712100, China.
College of Grassland Agriculture, Northwest A&F University, Yangling 712100, China; and College of Grassland, Resources and Environment, Inner Mongolia Agricultural University, Hohhot 010011, China.
Funct Plant Biol. 2024 Mar;51. doi: 10.1071/FP23210.
Soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE)-associated proteins are a class of transmembrane proteins involved in intracellular trafficking pathways. However, the functions of many SNARE domain-containing proteins remain unclear. We have previously identified a SNARE-associated gene in alfalfa (Medicago sativa ) KILLING ME SLOWLY1 (MsKMS1 ), which is involved in various abiotic stresses. In this study, we investigated the function of MsKMS1 in the seed germination of transgenic tobacco (Nicotiana tabacum ). Phylogenetic analysis showed that MsKMS1 was homologous to the SNARE-associated or MAPR component-related proteins of other plants. Germination assays revealed that MsKMS1 negatively regulated seed germination under normal, D-mannitol and abscisic acid-induced stress conditions, yet MsKMS1 -overexpression could confer enhanced heat tolerance in transgenic tobacco. The suppressive effect on germination in MsKMS1 -overexpression lines was associated with higher abscisic acid and salicylic acid contents in seeds. This was accompanied by the upregulation of abscisic acid biosynthetic genes (ZEP and NCED ) and the downregulation of gibberellin biosynthetic genes (GA20ox2 and GA20ox3 ). Taken together, these results suggested that MsKMS1 negatively regulated seed germination by increasing abscisic acid and salicylic acid contents through the expression of genes related to abscisic acid and gibberellin biosynthesis. In addition, MsKMS1 could improve heat tolerance during the germination of transgenic tobacco seeds.
可溶性 N-乙基马来酰亚胺敏感因子附着蛋白受体（SNARE）相关蛋白是一类参与细胞内运输途径的跨膜蛋白。然而，许多含有 SNARE 结构域的蛋白质的功能仍然不清楚。我们之前在紫花苜蓿（Medicago sativa）KILLING ME SLOWLY1（MsKMS1）中鉴定了一个与 SNARE 相关的基因，该基因参与了各种非生物胁迫。在本研究中，我们研究了 MsKMS1 在转基因烟草（Nicotiana tabacum）种子萌发中的功能。系统发育分析表明，MsKMS1 与其他植物的 SNARE 相关或 MAPR 组成相关蛋白同源。萌发实验表明，MsKMS1 在正常、甘露醇和脱落酸诱导的胁迫条件下负调控种子萌发，但 MsKMS1 的过表达可提高转基因烟草的耐热性。MsKMS1 过表达系对萌发的抑制作用与种子中较高的脱落酸和水杨酸含量有关。这伴随着脱落酸生物合成基因（ZEP 和 NCED）的上调和赤霉素生物合成基因（GA20ox2 和 GA20ox3）的下调。综上所述，这些结果表明 MsKMS1 通过表达与脱落酸和赤霉素生物合成相关的基因来增加脱落酸和水杨酸的含量，从而负调控种子萌发。此外，MsKMS1 可以提高转基因烟草种子萌发过程中的耐热性。
