Jeong Jin-Ju, Jin Yoo-Jeong, Ganesan Raja, Park Hee Jin, Min Byeong Hyun, Jeong Min Kyo, Yoon Sang Jun, Choi Mi Ran, Sharma Satya Priya, Jang You Jin, Min Uigi, Lim Jong-Hyun, Na Kyeong Min, Choi Jieun, Han Sang Hak, Ham Young Lim, Lee Do Yup, Kim Byung-Yong, Suk Ki Tae
Institute for Liver and Digestive Disease, College of Medicine, Hallym University, Chuncheon, Korea.
R&D Center, Chong Kun Dang Healthcare, Seoul, Republic of Korea.
Probiotics Antimicrob Proteins. 2024 Mar 12. doi: 10.1007/s12602-024-10232-4.
Diarrhea, a common gastrointestinal symptom in health problems, is highly associated with gut dysbiosis. The purpose of this study is to demonstrate the effect of multistrain probiotics (Sensi-Biome) on diarrhea from the perspective of the microbiome-neuron axis. Sensi-Biome (Lactiplantibacillus plantarum, Bifidobacterium animalis subsp. lactis, Lactobacillus acidophilus, Streptococcus thermophilus, Bifidobacterium bifidum, and Lactococcus lactis) was administered in a 4% acetic acid-induced diarrhea rat model at concentrations of 1 × 10 (G1), 1 × 10 (G2), and 1 × 10 CFU/0.5 mL (G3). Diarrhea-related parameters, inflammation-related cytokines, and stool microbiota analysis by 16S rRNA were evaluated. A targeted and untargeted metabolomics approach was used to analyze the cecum samples using liquid chromatography and orbitrap mass spectrometry. The stool moisture content (p < 0.001), intestinal movement rate (p < 0.05), and pH (p < 0.05) were significantly recovered in G3. Serotonin levels were decreased in the multistrain probiotics groups. The inflammatory cytokines, serotonin, and tryptophan hydroxylase expression were improved in the Sensi-Biome groups. At the phylum level, Sensi-Biome showed the highest relative abundance of Firmicutes. Short-chain fatty acids including butyrate, iso-butyrate, propionate, and iso-valeric acid were significantly modified in the Sensi-Biome groups. Equol and oleamide were significantly improved in the multistrain probiotics groups. In conclusion, Sensi-Biome effectively controls diarrhea by modulating metabolites and the serotonin pathway.
腹泻是健康问题中常见的胃肠道症状，与肠道菌群失调高度相关。本研究的目的是从微生物群-神经元轴的角度证明多菌株益生菌（Sensi-Biome）对腹泻的影响。在4%乙酸诱导的腹泻大鼠模型中，以1×10（G1）、1×10（G2）和1×10 CFU/0.5 mL（G3）的浓度给予Sensi-Biome（植物乳杆菌、动物双歧杆菌亚种乳酸亚种、嗜酸乳杆菌、嗜热链球菌、两歧双歧杆菌和乳酸乳球菌）。评估了腹泻相关参数、炎症相关细胞因子以及通过16S rRNA进行的粪便微生物群分析。采用靶向和非靶向代谢组学方法，使用液相色谱和轨道阱质谱对盲肠样本进行分析。G3组的粪便水分含量（p<0.001）、肠道蠕动率（p<0.05）和pH值（p<0.05）显著恢复。多菌株益生菌组的血清素水平降低。Sensi-Biome组的炎症细胞因子、血清素和色氨酸羟化酶表达得到改善。在门水平上，Sensi-Biome显示厚壁菌门的相对丰度最高。Sensi-Biome组中包括丁酸、异丁酸、丙酸和异戊酸在内的短链脂肪酸发生了显著变化。多菌株益生菌组的雌马酚和油酰胺显著改善。总之，Sensi-Biome通过调节代谢物和血清素途径有效控制腹泻。
