Mating Moritz, Sharbati Soroush, Einspanier Ralf
Institute of Veterinary Biochemistry, Freie Universität Berlin, Berlin, Germany.
Front Insect Sci. 2022 Mar 24;2:829869. doi: 10.3389/finsc.2022.829869. eCollection 2022.
Honeybees are important managed pollinators that perform important ecological and economic functions. In recent decades, the obligate ectoparasite severely affected survival of honeybees as it either feeds on hemolymph and fat bodies or acts as a vector for viruses. A common treatment against the varroa mite is formic acid, which has been used for many years by beekeepers. This treatment is known to be effective, but the therapeutic index is very narrow. Many beekeepers report negative effects of formic acid on bees, which include damage to brood, worker bee mortality, and queen loss. Little is yet known about the molecular mechanisms of formic acid detoxification in honeybees. Our previous study shows the upregulation of predicted 10-formyl tetrahydrofolate dehydrogenase (10-FTHFDH) transcripts in honeybees exposed to formic acid. Here, the predicted honeybee-specific 10-FTHFDH is recombinantly expressed, and its hydrolase and dehydrogenase activities are investigated. As a result, the enzyme shows similar dehydrogenase activity in comparison to known 10-FTHFDHs. This study provides further knowledge to better understand the detoxification mechanisms of formic acid in .
蜜蜂是重要的人工饲养传粉者，发挥着重要的生态和经济功能。近几十年来，这种专性体外寄生虫严重影响了蜜蜂的生存，因为它要么取食血淋巴和脂肪体，要么充当病毒载体。一种常见的防治瓦螨的方法是使用甲酸，养蜂人已经使用多年。已知这种处理方法有效，但治疗指数非常窄。许多养蜂人报告了甲酸对蜜蜂的负面影响，包括对幼虫的损害、工蜂死亡和蜂王损失。关于蜜蜂中甲酸解毒的分子机制，目前所知甚少。我们之前的研究表明，暴露于甲酸的蜜蜂中预测的10-甲酰四氢叶酸脱氢酶（10-FTHFDH）转录本上调。在此，对预测的蜜蜂特异性10-FTHFDH进行重组表达，并研究其水解酶和脱氢酶活性。结果表明，与已知的10-FTHFDH相比，该酶具有相似的脱氢酶活性。这项研究为更好地理解甲酸在蜜蜂中的解毒机制提供了更多知识。
