Centre for Plant Sciences, University of Leeds, Leeds LS2 9JT, UK.
Department of Molecular Genetics and Cell Biology, The University of Chicago, 929 East 57th Street GCIS 524W, Chicago, IL 60637, USA.
STAR Protoc. 2024 Jun 21;5(2):102944. doi: 10.1016/j.xpro.2024.102944. Epub 2024 Mar 11.
Understanding the generation, movement, uptake, and perception of mobile defense signals is key for unraveling the systemic resistance programs in flowering plants against pathogens. Here, we present a protocol for analyzing the movement and uptake of isotopically labeled signaling molecule azelaic acid (AZA) in Arabidopsis thaliana. We describe steps to assess C-AZA uptake into leaf discs and its movement from local to systemic tissues. We also detail the assay for uptake and movement of H-AZA from roots to the shoot. For complete details on the use and execution of this protocol, please refer to Cecchini et al..
了解移动防御信号的产生、移动、摄取和感知是揭示植物对病原体系统抗性程序的关键。在这里，我们提供了一种分析拟南芥中同位素标记信号分子壬二酸（AZA）移动和摄取的方案。我们描述了评估 C-AZA 进入叶圆片摄取及其从局部到系统组织移动的步骤。我们还详细说明了从根部到地上部摄取和移动 H-AZA 的测定。有关此方案使用和执行的完整详细信息，请参阅 Cecchini 等人的研究。
