Key Lab of Marine Environment and Ecology, Ministry of Education, Ocean University of China, Qingdao 266100, China; Shandong Provincial Key Laboratory of Marine Environment and Geological Engineering, Ocean University of China, Qingdao 266100, China.
Key Lab of Marine Environment and Ecology, Ministry of Education, Ocean University of China, Qingdao 266100, China.
J Hazard Mater. 2024 May 5;469:133983. doi: 10.1016/j.jhazmat.2024.133983. Epub 2024 Mar 8.
The transient chlorophenol shock under some emergency conditions might directly affect the pollutant removal of bioreactor. Therefore, the recovery of bioreactor performance after transient chlorophenol shock is a noteworthy issue. In the present research, the performance, antioxidant response, microbial succession and functional genes of sequencing batch reactor (SBR) were evaluated under transient 2,4,6-trichlorophenol (2,4,6-TCP) shock. The chemical oxygen demand (COD) and ammonia nitrogen (NH-N) removal efficiencies decreased sharply in the first 4 days after 60 mg/L 2,4,6-TCP shock for 24 h and gradually recovered to normal in the subsequent 8 days. The nitrogen removal rates and their corresponding enzymatic activities rapidly decreased after transient 2,4,6-TCP shock and then gradually increased to normal. The increase of antioxidant enzymatic activity, Cu-Zn SOD genes and Fe-Mn SOD genes contributed to the recovery of SBR performance. The abundance of genes encoding ammonia monooxygenase and hydroxylamine dehydrogenase decreased after transient 2,4,6-TCP shock, including amoA, amoC and nxrA. Thauera, Dechloromonas and Candidatus_Competibacter played key roles in the restorative process, which provided stable abundances of narG, norB , norC and nosZ. The results will deeply understand into the effect of transient 2,4,6-TCP shock on bioreactor performance and provide theoretical basis to build promising recoveries strategy of bioreactor performance.
在某些紧急情况下，短暂的氯酚冲击可能会直接影响生物反应器的污染物去除。因此，生物反应器在短暂氯酚冲击后的性能恢复是一个值得关注的问题。本研究在短暂的 2,4,6-三氯酚（2,4,6-TCP）冲击下评估了序批式生物反应器（SBR）的性能、抗氧化响应、微生物演替和功能基因。在 60mg/L 2,4,6-TCP 冲击 24 小时后，SBR 的化学需氧量（COD）和氨氮（NH-N）去除效率在最初的 4 天内急剧下降，随后在接下来的 8 天内逐渐恢复正常。氮去除率及其相应的酶活性在短暂的 2,4,6-TCP 冲击后迅速下降，然后逐渐恢复正常。抗氧化酶活性、Cu-Zn SOD 基因和 Fe-Mn SOD 基因的增加有助于 SBR 性能的恢复。短暂的 2,4,6-TCP 冲击后，编码氨单加氧酶和羟胺脱氢酶的基因丰度降低，包括 amoA、amoC 和 nxrA。Thauera、Dechloromonas 和 Candidatus_Competibacter 在恢复过程中发挥了关键作用，为 narG、norB、norC 和 nosZ 提供了稳定的丰度。研究结果将深入了解短暂的 2,4,6-TCP 冲击对生物反应器性能的影响，并为构建生物反应器性能的有前途的恢复策略提供理论依据。
