Yunnan Center for Disease Control and Prevention, No. 158 Dongsi Road, Kunming 650022, People's Republic of China.
Kunming Center for Disease Control and Prevention, No. 4 Ziyun Road, Kunming 650228, People's Republic of China.
J Appl Microbiol. 2024 Mar 1;135(3). doi: 10.1093/jambio/lxae060.
Enteroviruses are significant human pathogens associated with a range of mild to severe diseases. This study aims to understand the diversity and genetic characterization of enteroviruses circulated in southwest China's border cities by using environmental surveillance.
A total of 96 sewage samples were collected in three border cities and a port located in Yunnan Province, China from July 2020 to June 2022. After cell culture and VP1 sequencing, a total of 590 enterovirus isolates were identified, belonging to 21 types. All PV strains were Sabin-like with ≤6 nucleotide mutations in the VP1 coding region. Echovirus 6, echovirus 21 (a rare serotype in previous studies), and coxsackievirus B5 were the predominant serotypes, which accounted for 21.19%, 18.31%, and 13.39% of the total isolates, respectively. The prevalence of the common serotypes varied across different border cities and periods. Phylogenetic analysis revealed the presence of multiple evolutionary lineages for E21, E6, and E30, some of which formed distinct branches.
High diversity of enteroviruses and distinct lineages of predominant serotypes circulated in southwest China's border cities.
肠道病毒是与一系列轻度至重度疾病相关的重要人类病原体。本研究旨在通过环境监测了解中国西南边境城市流行的肠道病毒的多样性和遗传特征。
本研究于 2020 年 7 月至 2022 年 6 月在云南省的三个边境城市和一个港口共采集了 96 份污水样本。经过细胞培养和 VP1 测序，共鉴定出 590 株肠道病毒分离株，属于 21 种类型。所有 PV 株均为 Sabin 样株，VP1 编码区的核苷酸突变≤6。肠病毒 6、肠病毒 21（以前研究中罕见的血清型）和柯萨奇病毒 B5 是主要的血清型，分别占总分离株的 21.19%、18.31%和 13.39%。常见血清型的流行情况在不同的边境城市和时期有所不同。系统进化分析显示，E21、E6 和 E30 存在多种进化谱系，其中一些形成了不同的分支。
中国西南边境城市流行的肠道病毒具有高度多样性和优势血清型的独特谱系。
