Bi Jingran, Yao Qiaoli, Zhang Gongliang, Hou Hongman
School of Food Science and Technology, Dalian Polytechnic University, No. 1, Qinggongyuan, Ganjingzi District, Dalian 116034, China.
Liaoning Key Lab for Aquatic Processing Quality and Safety, No. 1, Qinggongyuan, Ganjingzi District, Dalian 116034, China.
Foods. 2024 Feb 24;13(5):688. doi: 10.3390/foods13050688.
, a specific spoilage microorganism, has a strong capacity to destroy food protein and lead to spoilage. The aim of this study was to evaluate the phase-dependent regulation of lux-type genes on the spoilage characteristics of H4. The auto-inducer synthase gene and a regulatory gene of the quorum sensing systems in H4 were knocked out to construct the mutant phenotypes. On this basis, the research found that the and genes had a strong positive influence on not only flagella-dependent swimming ability and biofilm formation but also the production of putrescine and cadaverine. The gene could downregulate putrescine production. The maximum accumulation of putrescine in wild type, Δ, Δ and Δ were detected at 24 h, reaching up to 695.23 mg/L, 683.02 mg/L, 776.30 mg/L and 724.12 mg/L, respectively. However, the and genes have a potential positive impact on the production of cadaverine. The maximum concentration of cadaverine produced by wild type, Δ, Δ and Δ were 252.7 mg/L, 194.5 mg/L, 175.1 mg/L and 154.2 mg/L at 72 h. Moreover, the self-organizing map analysis revealed the phase-dependent effects of two genes on spoilage properties. The gene played a major role in the lag phase, while the gene mainly acted in the exponential and stationary phases. Therefore, the paper provides valuable insights into the spoilage mechanisms of H4.
一种特定的腐败微生物具有很强的破坏食物蛋白质并导致食物变质的能力。本研究的目的是评估lux型基因对H4腐败特性的阶段依赖性调控。敲除H4群体感应系统中的自诱导物合成酶基因和一个调控基因以构建突变表型。在此基础上，研究发现该基因和基因不仅对依赖鞭毛的游动能力和生物膜形成有很强的积极影响，而且对腐胺和尸胺的产生也有积极影响。基因可以下调腐胺的产生。野生型、Δ、Δ和Δ中腐胺的最大积累量在24小时时被检测到，分别达到695.23毫克/升、683.02毫克/升、776.30毫克/升和724.12毫克/升。然而，该基因和基因对尸胺的产生有潜在的积极影响。野生型、Δ、Δ和Δ在72小时时产生的尸胺最大浓度分别为252.7毫克/升、194.5毫克/升、175.1毫克/升和154.2毫克/升。此外，自组织映射分析揭示了两个基因对腐败特性的阶段依赖性影响。基因在延滞期起主要作用，而基因主要在指数期和稳定期起作用。因此，本文为H4的腐败机制提供了有价值的见解。
