Tavares Flávia Dos Santos, Martins Cesar, Delella Flávia Karina, Nascimento Luís Adriano Santos do, Rodrigues Angélica Lúcia Figueiredo, Moreira Sávia, Cardoso Adauto Lima, Noronha Renata Coelho Rodrigues
Laboratório de Genética e Biologia Celular, Centro de Estudos Avançados da Biodiversidade, Instituto de Ciências Biológicas, Universidade Federal do Pará, Belém 66075-110, PA, Brazil.
Department of Structural and Functional Biology, Institute of Biosciences at Botucatu, Sao Paulo State University-UNESP, Botucatu 18618-689, SP, Brazil.
Animals (Basel). 2024 Feb 22;14(5):686. doi: 10.3390/ani14050686.
The vulnerable status of the Amazon manatee, , indicates the need to seek measures to guarantee its conservation. In this context, the cultivation of cells in vitro is a strategy that should at least guarantee the preservation of their genetic material. Thus, we established for the first time a primary culture of Amazonian manatee fibroblasts (TINsf) from a skin biopsy of a young male. Karyotypic analysis of the 3rd, 7th, and 12th passages confirmed the taxonomic identity of the species (2n = 56/NF = 92) and indicated that this culture presents genomic stability. Gene and protein expression of vimentin at the 13th passage show the predominant presence of fibroblasts in TINsf. To test the cell line's responsiveness to materials and demonstrate a possible application of this culture, it was exposed to andiroba seed oil (ASO), and its viability and proliferative capacity were evaluated. ASO demonstrated toxic effects at the highest concentrations and longest exposure times tested, reproducing results observed in human cultures, indicating the applicability of TINsf in toxicological and biotechnological studies. After cryopreservation, the TINsf line maintained its proliferative potential, indicating the establishment of a new culture available for future studies.
亚马逊海牛的濒危状态表明需要寻求保障其保护的措施。在此背景下，体外细胞培养是一种至少应能保障其遗传物质保存的策略。因此，我们首次从一头年轻雄性亚马逊海牛的皮肤活检样本中建立了亚马逊海牛成纤维细胞原代培养物（TINsf）。对第3、7和12代进行的核型分析证实了该物种的分类身份（2n = 56/NF = 92），并表明这种培养物具有基因组稳定性。第13代波形蛋白的基因和蛋白表达表明TINsf中主要存在成纤维细胞。为了测试该细胞系对物质的反应能力并证明这种培养物的可能应用，将其暴露于巴西香脂树籽油（ASO）中，并评估其活力和增殖能力。在测试的最高浓度和最长暴露时间下，ASO表现出毒性作用，重现了在人类培养物中观察到的结果，表明TINsf在毒理学和生物技术研究中的适用性。冷冻保存后，TINsf细胞系保持了其增殖潜力，表明建立了一种可用于未来研究的新培养物。
