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猪圆环病毒4型的核定位信号影响病毒衣壳的亚细胞定位及病毒样颗粒的产生。

The Nuclear Localization Signal of Porcine Circovirus Type 4 Affects the Subcellular Localization of the Virus Capsid and the Production of Virus-like Particles.

作者信息

Zheng Jiawei, Li Nan, Li Xue, Han Yaqi, Lv Xinru, Zhang Huimin, Ren Linzhu

机构信息

College of Animal Sciences, Key Lab for Zoonoses Research, Ministry of Education, Jilin University, 5333 Xi'an Road, Changchun 130062, China.

Changchun Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 666 Liuying West Road, Changchun 130122, China.

出版信息

Int J Mol Sci. 2024 Feb 20;25(5):2459. doi: 10.3390/ijms25052459.

Abstract

Porcine circovirus 4 (PCV4) is a newly identified virus belonging to PCV of the family, the genus. We previously found that PCV4 is pathogenic in vitro, while the virus's replication in cells is still unknown. In this study, we evaluated the N-terminal of the PCV4 capsid (Cap) and identified an NLS at amino acid residues 4-37 of the N-terminus of the PCV4 Cap, RSRYSRRRRNRRNQRRRGLWPRASRRRYRWRRKN. The NLS was further divided into two fragments (NLS-A and NLS-B) based on the predicted structure, including two α-helixes, which were located at RSRYSRRRRNRRNQRR and PRASRRRYRWRRK, respectively. Further studies showed that the NLS, especially the first α-helixes formed by the NLS-A fragment, determined the nuclear localization of the Cap protein, and the amino acid RSRY in the NLS of the PCV4 Cap was the critical motif affecting the VLP packaging. These results will provide a theoretical basis for elucidating the infection mechanism of PCV4 and developing subunit vaccines based on VLPs.

摘要

猪圆环病毒4型(PCV4)是一种新发现的病毒,属于圆环病毒科圆环病毒属。我们之前发现PCV4在体外具有致病性,但其在细胞中的复制情况仍不清楚。在本研究中,我们对PCV4衣壳蛋白(Cap)的N端进行了评估,并在PCV4 Cap N端的第4至37个氨基酸残基处鉴定出一个核定位信号(NLS),即RSRYSRRRRNRRNQRRRGLWPRASRRRYRWRRKN。根据预测结构,该NLS进一步分为两个片段(NLS-A和NLS-B),包括两个α螺旋,分别位于RSRYSRRRRNRRNQRR和PRASRRRYRWRRK处。进一步研究表明,该NLS,尤其是由NLS-A片段形成的第一个α螺旋,决定了Cap蛋白的核定位,并且PCV4 Cap NLS中的氨基酸RSRY是影响病毒样颗粒(VLP)包装的关键基序。这些结果将为阐明PCV4的感染机制以及开发基于VLP的亚单位疫苗提供理论依据。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a5df/10930891/7442e5d88cba/ijms-25-02459-g001.jpg

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