National Key Laboratory of Veterinary Public Health and Safety, College of Veterinary Medicine, China Agricultural University, Beijing 100083, China.
Key Laboratory of Animal Epidemiology of the Ministry of Agriculture, College of Veterinary Medicine, China Agricultural University, Beijing 100083, China.
Int J Mol Sci. 2024 Feb 21;25(5):2493. doi: 10.3390/ijms25052493.
Iron is an indispensable nutrient for the survival of ; however, excessive amounts can lead to toxicity. The parasite must overcome the host's "nutritional immunity" barrier and compete with the host for iron. Since can infect most nucleated cells, it encounters increased iron stress during parasitism. This study assessed the impact of iron stress, encompassing both iron depletion and iron accumulation, on the growth of . Iron accumulation disrupted the redox balance of while enhancing the parasite's ability to adhere in high-iron environments. Conversely, iron depletion promoted the differentiation of tachyzoites into bradyzoites. Proteomic analysis further revealed proteins affected by iron depletion and identified the involvement of phosphotyrosyl phosphatase activator proteins in bradyzoite formation.
铁是生存必需的营养物质;然而，过量的铁会导致毒性。寄生虫必须克服宿主的“营养免疫”障碍，并与宿主争夺铁。由于可以感染大多数有核细胞，因此在寄生过程中会遇到增加的铁应激。本研究评估了铁应激（包括缺铁和铁积累）对的生长的影响。铁积累破坏了的氧化还原平衡，同时增强了寄生虫在高铁环境中的粘附能力。相反，缺铁促进了速殖子向缓殖子的分化。蛋白质组学分析进一步揭示了受缺铁影响的蛋白质，并确定了磷酸酪氨酸磷酸酶激活蛋白在缓殖子形成中的参与。
