Liu Xiaofan, Zhao Huihui, Xie Jiatao, Fu Yanping, Li Bo, Yu Xiao, Chen Tao, Lin Yang, Jiang Daohong, Cheng Jiasen
State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, China.
The Provincial Key Laboratory of Plant Pathology of Hubei Province, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, China.
Int J Mol Sci. 2024 Feb 26;25(5):2693. doi: 10.3390/ijms25052693.
Phytopathogenic fungi normally secrete large amounts of CWDEs to enhance infection of plants. In this study, we identified and characterized a secreted glycosyl hydrolase 5 family member in (SsGH5, Glycosyl Hydrolase 5). was significantly upregulated during the early stages of infection. Knocking out did not affect the growth and acid production of but resulted in decreased glucan utilization and significantly reduced virulence. In addition, expressing SsGH5 became more susceptible to necrotrophic pathogens and basal immune responses were inhibited in these plants. Remarkably, the lost virulence of the mutants was restored after inoculating onto transgenic . In summary, these results highlight that suppresses the immune responses of through secreting SsGH5, and thus exerts full virulence for successful infection.
植物病原真菌通常会分泌大量细胞壁降解酶以增强对植物的感染。在本研究中，我们鉴定并表征了一种在[具体名称未给出]中的分泌型糖基水解酶5家族成员（SsGH5，糖基水解酶5）。在感染早期，[具体名称未给出]显著上调。敲除[具体名称未给出]并不影响[具体名称未给出]的生长和产酸，但导致葡聚糖利用减少且毒力显著降低。此外，过表达SsGH5的[具体名称未给出]对坏死营养型病原体更敏感，并且这些植物中的基础免疫反应受到抑制。值得注意的是，在接种到[具体名称未给出]转基因植株上后，[具体名称未给出]突变体丧失的毒力得以恢复。总之，这些结果表明[具体名称未给出]通过分泌SsGH5抑制[具体名称未给出]的免疫反应，从而发挥完全毒力以成功感染。
