Nordstrom Hayley R, Griffith Marissa P, Rangachar Srinivasa Vatsala, Wallace Nathan R, Li Anna, Cooper Vaughn S, Shields Ryan K, Van Tyne Daria
Division of Infectious Diseases, University of Pittsburgh School of Medicine, Pittsburgh, PA 15213, USA.
Department of Microbiology and Molecular Genetics, University of Pittsburgh School of Medicine, Pittsburgh, PA 15213, USA.
Cells. 2024 Feb 29;13(5):428. doi: 10.3390/cells13050428.
spp. are often resistant to antibiotics, and infections with these organisms are difficult to treat. A potential alternative treatment for spp. infections is bacteriophage (phage) therapy; however, it can be difficult to locate phages that target these bacteria. Prophages incorporated into the bacterial genome have been identified within spp. and may represent a source of useful phages for therapy. Here, we investigate whether prophages within spp. clinical isolates can kill conspecific and heterospecific isolates. Thirty-two spp. isolates were induced for prophage release, and harvested phages were tested for lytic activity against the same 32 isolates. Temperate phages were passaged and their host ranges were determined, resulting in four unique phages of prophage origin that showed different ranges of lytic activity. We also analyzed the prophage content of 35 spp. clinical isolate genomes and identified several prophages present in the genomes of multiple isolates of the same species. Finally, we observed that isolates were more phage-susceptible than isolates. Overall, our findings suggest that prophages present within spp. genomes are a potentially useful starting point for the isolation and development of novel phages for use in phage therapy.
[细菌名称]通常对抗生素具有抗性，并且这些生物体引起的感染难以治疗。针对[细菌名称]感染的一种潜在替代疗法是噬菌体疗法；然而，很难找到靶向这些细菌的噬菌体。已在[细菌名称]中鉴定出整合到细菌基因组中的前噬菌体，它们可能代表了用于治疗的有用噬菌体来源。在此，我们研究[细菌名称]临床分离株中的前噬菌体是否能杀死同种和异种分离株。诱导32株[细菌名称]分离株释放前噬菌体，并测试收获的噬菌体对相同32株分离株的裂解活性。对温和噬菌体进行传代并确定其宿主范围，得到了四种源自前噬菌体的独特噬菌体，它们表现出不同范围的裂解活性。我们还分析了35株[细菌名称]临床分离株基因组的前噬菌体含量，并在同一物种的多个分离株基因组中鉴定出几种前噬菌体。最后，我们观察到[细菌名称]分离株比[另一种细菌名称]分离株对噬菌体更敏感。总体而言，我们的研究结果表明，[细菌名称]基因组中存在的前噬菌体是分离和开发用于噬菌体疗法的新型噬菌体的潜在有用起点。
