Sun Yanmei, Zhao Na, Sun Hongjian, Xu Shan, Lu Yiwen, Xi Haojie, Guo Zhenfei, Shi Haifan
Key Laboratory of State Forestry and Grassland Administration on Grass Germplasm Resources Innovation and Utilization in the Middle and Lower Reaches of the Yangtze River, College of Grassland Science, Nanjing Agricultural University, Nanjing 210095, China.
Plants (Basel). 2024 Mar 3;13(5):714. doi: 10.3390/plants13050714.
Common vetch ( L.) is an important annual diploid leguminous forage. In the present study, transcriptomic profiling in common vetch in response to salt stress was conducted using a salt-tolerant line (460) and a salt-sensitive line (429). The common responses in common vetch and the specific responses associated with salt tolerance in 460 were analyzed. Several KEGG (Kyoto Encyclopedia of Genes and Genomes) pathways, including plant hormone and MAPK (mitogen-activated protein kinase) signaling, galactose metabolism, and phenylpropanoid phenylpropane biosynthesis, were enriched in both lines, though some differentially expressed genes (DEGs) showed distinct expression patterns. The roots in 460 showed higher levels of lignin than in 429. α-linolenic acid metabolism, carotenoid biosynthesis, the photosynthesis-antenna pathway, and starch and sucrose metabolism pathways were specifically enriched in salt-tolerant line 460, with higher levels of accumulated soluble sugars in the leaves. In addition, higher transcript levels of genes involved in ion homeostasis and reactive oxygen species (ROS) scavenging were observed in 460 than in 429 in response to salt stress. The transcriptomic analysis in common vetch in response to salt stress provides useful clues for further investigations on salt tolerance mechanism in the future.
普通野豌豆（Vicia sativa L.）是一种重要的一年生二倍体豆科牧草。在本研究中，使用耐盐品系（460）和盐敏感品系（429）对普通野豌豆响应盐胁迫进行了转录组分析。分析了普通野豌豆的共同响应以及460中与耐盐性相关的特异性响应。包括植物激素和丝裂原活化蛋白激酶（MAPK）信号传导、半乳糖代谢以及苯丙烷类生物合成在内的几个京都基因与基因组百科全书（KEGG）途径在两个品系中均有富集，尽管一些差异表达基因（DEG）表现出不同的表达模式。460的根中木质素水平高于429。α-亚麻酸代谢、类胡萝卜素生物合成、光合作用天线途径以及淀粉和蔗糖代谢途径在耐盐品系460中特异性富集，其叶片中积累的可溶性糖水平更高。此外，响应盐胁迫时，460中参与离子稳态和活性氧（ROS）清除的基因转录水平高于429。普通野豌豆响应盐胁迫的转录组分析为未来进一步研究耐盐机制提供了有用线索。
