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幼虫作为研究毒力和抗菌耐药性的新型模型。

larvae as a novel model for the study of virulence and antimicrobial resistance.

作者信息

Rakovitsky Nadya, Temkin Elizabeth, Hameir Amichay, Lurie-Weinberger Mor, Keren-Paz Alona, Carmeli Yehuda

机构信息

National Institute for Antibiotic Resistance and Infection Control, Israel Ministry of Health, Tel Aviv, Israel.

School of Medicine, Tel Aviv University, Tel Aviv, Israel.

出版信息

Front Microbiol. 2024 Feb 27;15:1375787. doi: 10.3389/fmicb.2024.1375787. eCollection 2024.

DOI:10.3389/fmicb.2024.1375787
PMID:38476953
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10927975/
Abstract

The use of mammalian models for testing of bacterial virulence raises ethical concerns and is expensive and time-consuming. As an alternative, non-mammalian models are sought. larvae have been used as a model to study several bacterial pathogens. However, their maintenance is challenging, and commercial supply is low. In this study, we aimed to establish the larvae as an alternative non-mammalian model for the evaluation of the pathogenicity and antimicrobial susceptibility of . We infected with strains and determined the optimal temperature and inoculum. To visualize the bacterial distribution within the larvae, hematoxylin and eosin (H&E) staining was performed. Next, a survival model of infected larvae was established, and virulence was compared between strains. The effect of antimicrobial treatment in relation to antibiotic susceptibility was studied. Our results demonstrate that can be used as a model system for studies of .

摘要

使用哺乳动物模型来测试细菌毒力引发了伦理问题，而且成本高昂、耗时费力。作为替代方案，人们在寻找非哺乳动物模型。幼虫已被用作研究多种细菌病原体的模型。然而，饲养幼虫具有挑战性，且商业供应不足。在本研究中，我们旨在确立该幼虫作为一种替代非哺乳动物模型，用于评估[具体细菌名称]的致病性和抗菌药敏性。我们用[具体细菌名称]菌株感染幼虫，并确定了最佳温度和接种量。为了观察细菌在幼虫体内的分布情况，进行了苏木精和伊红（H&E）染色。接下来，建立了感染幼虫的生存模型，并比较了不同菌株之间的毒力。研究了抗菌治疗与抗生素敏感性相关的效果。我们的结果表明，该幼虫可作为用于[具体细菌名称]研究的模型系统。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5863/10927975/e197980d8d4f/fmicb-15-1375787-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5863/10927975/35e6000ef276/fmicb-15-1375787-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5863/10927975/2e29b5688a3f/fmicb-15-1375787-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5863/10927975/52ac37b680a1/fmicb-15-1375787-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5863/10927975/00ecd7b3b414/fmicb-15-1375787-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5863/10927975/e197980d8d4f/fmicb-15-1375787-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5863/10927975/35e6000ef276/fmicb-15-1375787-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5863/10927975/2e29b5688a3f/fmicb-15-1375787-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5863/10927975/52ac37b680a1/fmicb-15-1375787-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5863/10927975/00ecd7b3b414/fmicb-15-1375787-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5863/10927975/e197980d8d4f/fmicb-15-1375787-g005.jpg

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