Department of Surgery, NUTRIM School of Nutrition and Translational Research in Metabolism, Maastricht University, Maastricht, The Netherlands.
Proteomics. 2024 Jun;24(12-13):e2300160. doi: 10.1002/pmic.202300160. Epub 2024 Mar 13.
Intestinal ischemia-reperfusion injury (IR) is implicated in various clinical conditions and causes damage to the intestinal epithelium resulting in intestinal barrier loss. This presents a substantial clinical challenge, emphasizing the importance of gaining a comprehensive understanding of molecular events to aid in the identification of novel therapeutic targets. This review systematically explores the extent to which omics technologies-transcriptomics, proteomics, metabolomics, and metagenomics-have already contributed to deciphering the molecular mechanisms contributing to intestinal IR injury, in in vivo and in vitro animal and human models, and in clinical samples. Recent breakthroughs involve applying omics methodologies on exosomes, organoids, and single cells, shedding light on promising avenues and valuable targets to reduce intestinal IR injury. Future directions aimed at expediting clinical translation are discussed as well and include multi-omics data integration to facilitate the identification of key regulatory nodes driving intestinal IR injury and advancing human organoid models based on the novel insights by single-cell omics technologies, offering hope for clinical application of therapeutic strategies in the years to come.
肠缺血再灌注损伤（IR）与多种临床情况有关，可导致肠上皮损伤，进而使肠道屏障丧失。这是一个重大的临床挑战，强调了全面了解分子事件的重要性，以帮助确定新的治疗靶点。本综述系统地探讨了组学技术（转录组学、蛋白质组学、代谢组学和宏基因组学）在多大程度上有助于解析参与肠 IR 损伤的分子机制，包括在体内和体外动物和人类模型以及临床样本中的作用。最近的突破涉及在细胞外囊泡、类器官和单细胞上应用组学方法，为减少肠 IR 损伤提供了有前途的途径和有价值的靶点。本文还讨论了旨在加速临床转化的未来方向，包括多组学数据的整合，以促进识别关键调控节点，推动基于单细胞组学技术的新型人类类器官模型的发展，为未来治疗策略的临床应用带来希望。
