体外mRNA前体的反式剪接

Trans splicing of mRNA precursors in vitro.

作者信息

Konarska M M, Padgett R A, Sharp P A

出版信息

Cell. 1985 Aug;42(1):165-71. doi: 10.1016/s0092-8674(85)80112-4.

Abstract

Two exon segments from two separate RNA molecules can be joined in a trans splicing process. In trans splicing reactions, an RNA molecule containing an exon, a 5' splice site, and adjacent intron sequences was mixed with an RNA molecule containing an exon, a 3' splice site, and adjacent intron sequences. The efficiency of trans splicing of these two RNAs increased if the two termini of the intervening sequences were paired in a short RNA duplex. However, trans splicing of two RNA molecules with no significant complementarity was also observed. These results strongly suggest that significant secondary structures within intervening sequences could affect the splicing of flanking exons. Similarly, RNAs that are complementary to segments within the intervening sequences could potentially regulate the selection of splice sites. Finally, some organisms might use trans splicing to distribute a single exon to many different mRNAs.

摘要

来自两个独立RNA分子的两个外显子片段可在反式剪接过程中连接。在反式剪接反应中，将一个包含外显子、5'剪接位点和相邻内含子序列的RNA分子与一个包含外显子、3'剪接位点和相邻内含子序列的RNA分子混合。如果中间序列的两个末端在一个短RNA双链体中配对，这两个RNA的反式剪接效率会提高。然而，也观察到两个没有明显互补性的RNA分子的反式剪接。这些结果有力地表明，中间序列内的显著二级结构可能会影响侧翼外显子的剪接。同样，与中间序列内的片段互补的RNA可能会潜在地调节剪接位点的选择。最后，一些生物体可能利用反式剪接将单个外显子分布到许多不同的mRNA中。