College of Engineering, China Agricultural University, Beijing, 100083, People's Republic of China.
Department of Civil and Environmental Engineering, Virginia Tech, Arlington, VA, 22202, USA.
Appl Biochem Biotechnol. 2024 Oct;196(10):7104-7127. doi: 10.1007/s12010-024-04900-y. Epub 2024 Mar 14.
Whether advanced biological waste treatment technologies, such as hydrothermal pretreatment (HTP) integrated anaerobic digestion (AD), could enhance the removal of different antibiotics remains unclear. This study investigated the outcome of antibiotics and methane productivity during pig manure treatment via HTP, AD, and HTP + AD. Results showed improved removal efficiency of sulfadiazine (SDZ), oxytetracycline (OTC), and enrofloxacin (ENR) with increased HTP temperatures (70, 90, 120, 150, and 170 °C). OTC achieved the highest removal efficiency of 86.8% at 170 °C because of its high sensitivity to heat treatment. For AD, SDZ exhibited resistance with a removal efficiency of 52.8%. However, OTC and ENR could be removed completely within 30 days. When HTP was used prior to AD, OTC and ENR could achieve complete removal. However, residual SDZ levels reduced to 20% and 16% at 150 and 170 °C, respectively. The methanogenic potential showed an overall upward trend as the HTP temperature increased. Microbial analysis revealed the antibiotics-induced enrichment of specific microorganisms during AD. Firmicutes were the dominant bacterial phylum, with their abundance positively correlated with the addition of antibiotics. Methanobacterium and Methanosarcina emerged as the dominant archaea that drove methane production during AD. Thus, HTP can be a potential pretreatment before AD to reduce antibiotic-related risks in manure waste handling.
高级生物废物处理技术，如热水预处理（HTP）与厌氧消化（AD）集成，是否能提高不同抗生素的去除率仍不清楚。本研究通过 HTP、AD 和 HTP+AD 处理猪粪，考察了抗生素的去除和甲烷生产力的结果。结果表明，随着 HTP 温度（70、90、120、150 和 170°C）的升高，磺胺嘧啶（SDZ）、土霉素（OTC）和恩诺沙星（ENR）的去除效率得到提高。由于 OTC 对热处理高度敏感，其去除效率高达 86.8%。对于 AD，SDZ 表现出抗性，去除效率为 52.8%。然而，OTC 和 ENR 可以在 30 天内完全去除。当 HTP 在 AD 之前使用时，OTC 和 ENR 可以完全去除。然而，在 150 和 170°C 下，残留的 SDZ 水平分别降低到 20%和 16%。随着 HTP 温度的升高，产甲烷潜力呈总体上升趋势。微生物分析表明，抗生素在 AD 过程中诱导了特定微生物的富集。厚壁菌门是主要的细菌门，其丰度与抗生素的添加呈正相关。甲烷杆菌属和甲烷八叠球菌属成为 AD 过程中驱动甲烷产生的主要古菌。因此，HTP 可以作为 AD 前的潜在预处理方法，以降低粪便废物处理中抗生素相关风险。
